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GSK3A
CRISPR
in HepG2
Control:
NT-BGHcLV14
General Information
Experiment Information
Sequencing Information
Data Submission
Files
General Information
RBP
GSK3A
Cell_Line
HepG2
Method
CRISPR
Exp_Name
GSK3A-BGHcLV14-HepG2
ENCODE_series_ID
ENCSR230NEZ
Batch_ID
BGHcLV14
Pool ID
Pool-190423
Local_Set_Name
set42
ENCODE_KD_Exp_ID
ENCSR300RMY
ENCODE_CN_Exp_ID
ENCSR750CPT
Rep1
GSK3A-BGHcLV14-41
Rep2
GSK3A-BGHcLV14-42
CN1
NT-BGHcLV14-1
CN2
NT-BGHcLV14-2
Rep1_qPCR
46.9
Rep2_qPCR
40.8
Rep1_WB
60.6
Rep2_WB
65.3
Antibody Cat#
MA3-038
Antibody Lot#
Antibody DCC ID
ENCAB957NOP
Status
Released
Project
ENCODE4
ID
174
Experiment Information
Western Blot
Experiment Status
Experiment Information (Status:
NotSatisfied
)
BGHcLV14-43
BGHcLV14-44
idx
398
399
TRCN#_or_BGC#
BGC#0000324
BGC#0000324
shRNA_or_gRNA_sequence
CTCATTTGGGGTCGTGTACC
CTCATTTGGGGTCGTGTACC
PAM
agg
agg
Name
GSK3_93
GSK3_93
Sample_ID
BGHcLV14-43
BGHcLV14-44
transduction_Date
12/5/18
12/5/18
days
D6
D6
RBP_name
GSK3A
GSK3A
qPCR_result
13.0
6.2
Ave_qPCR
9.6
RT-qPCR_primer-F
gagctgcagatcatgcgtaa
RT-qPCR_primer-R
gcacatattccagcaccaga
protein_conc
3940
4519
WB_result
47.9
49.4
Ave_WB
48.7
WB_DONE_date
1/4/19,2/15/19
MW
51, 47KD
IP
mouse
antibody_Cat#
MA3-038
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM
85
0
Rep2_TPM
70
0
Action
Library_start_date
repeat_library
Note
ID
6543
6544
Western Blot
Western Blot info was not avaliable
Experiment Status
BGHcLV14-43
BGHcLV14-44
Sample_ID
BGHcLV14-43
BGHcLV14-44
Sample Name
Sample Name
RBP
GSK3A
GSK3A
Cell_Line
HepG2
HepG2
Exp UID
Status
NotSatisfied
NotSatisfied
Status_date
2019-08-10
2019-08-10
Project
ENCODE4
ENCODE4
Note
ID
3023
3024
Library-Prep
Sequencing
Library-Prep Information
Library info was not avaliable
Sequencing Information
Sequencing info was not avaliable
Data Submission Information
DCC submission information was not avaliable
File Information
Files of the experiment was not avaliable in the database
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