WDR6 RNA-Seq in 293T

Experiment Information (Status: Released)

	BG293LV06-89	BG293LV06-90
idx	0	0
TRCN#_or_BGC#	TRCN0000353745	TRCN0000353745
shRNA_or_gRNA_sequence	CCGGCAATCGGCATCGGATGGTTAACTCGAGTTAACCATCCGATGCCGATTGTTTTTG	CCGGCAATCGGCATCGGATGGTTAACTCGAGTTAACCATCCGATGCCGATTGTTTTTG
PAM
Name	WDR6	WDR6
Sample_ID	BG293LV06-89	BG293LV06-90
transduction_Date	9/5/24	9/5/24
days	D4	D4
RBP_name	WDR6	WDR6
qPCR_result	69.2	72.6
Ave_qPCR	70.9
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc	0	0
WB_result
Ave_WB
WB_DONE_date
MW
IP
antibody_Cat#
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM	0	0
Rep2_TPM	0	0
Action	Ready	Ready
Library_start_date
repeat_library
Note
ID	12224	12225

Western Blot Western Blot info was not avaliable

Experiment Status

	BG293LV06-89	BG293LV06-90
Sample_ID	BG293LV06-89	BG293LV06-90
Sample Name
Sample Name	WDR6-BG293LV06-89B	WDR6-BG293LV06-90B
RBP	WDR6	WDR6
Cell_Line	293T	293T
Exp UID
Status	Released	Released
Status_date	2025-04-21	2025-04-21
Project	ENCORE2	ENCORE2
Note
ID	13526	13527

Library-Prep
Sequencing

Library-Prep Information

	BG293LV06-89	BG293LV06-90
Sample #	89	90
Sample Name	WDR6-BG293LV06-89B	WDR6-BG293LV06-90B
Sample_Name_Alias
Index Well Position	A05	B05
Index_table	IDT_UniqueDualIndex_96_V2	IDT_UniqueDualIndex_96_V2
LibPrep_date	2024-10-02	2024-10-02
Lib_ID	Lib-241002	Lib-241002
Tecan_Location
Tecan
Tecan_date
Size_bp	273	277
Peak_Molarity	105.00	91.60
libSampleQC_DNA_Well	DNA_Library_set72/set1/A5.png	DNA_Library_set72/set1/B5.png
RIN	10.0	9.8
libSample_RNA_Well	RNA_Library_set72/set1/A5.png	RNA_Library_set72/set1/B5.png
SampleQC_method	TapeStation_2022	TapeStation_2022
SampleQC_date	2024-10-04	2024-10-04
Sample_ID	BG293LV06-89	BG293LV06-90
RBP	WDR6	WDR6
Batch_ID	BG293LV06	BG293LV06
WB_result	0.000	0.000
Library Description	TruSeq mRNA	TruSeq mRNA
Repeat_Library_Suffix	B	B
Lib_Status	SendToSequence	SendToSequence
Project	ENCORE2	ENCORE2
ID	4514	4515

Sequencing Information

	BG293LV06-89	BG293LV06-90
Sample_ID	BG293LV06-89	BG293LV06-90
Sample Name	WDR6-BG293LV06-89B	WDR6-BG293LV06-90B
Pool ID	Pool-241004	Pool-241004
LocalServer_folder	set72	set72
total_reads	25,969,173	36,489,963
total_aligned_reads	23,421,278	32,709,436
unique_aligned_reads	21,892,316	30,638,153
percent_uniqueAligned	0.84301	0.83963
correlation_replicates	0.981294	0.981294
spikein_reads	111,982	186,382
percent_spikeins	0.00431	0.00511
original_ReadLength	101	101
QC_Status	Submitted	Submitted
ID	3083	3084

Data Submission Information DCC submission information was not avaliable

File Information

file_name	file_path	file_format	output_type	assay_type	lab	Sample ID	platform	read_length	run_type	paired_end	assembly	genome_annotation	derived_from	controlled_by	Project	FILE_ID
WDR6-BG293LV06-89B_S41_L001_R1_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set72/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3083	NovaSeq6000	100	paired-ended	1				NT-BG293LV06-1Bmerged_S9_L001_R1_001.filtered.trimmed.paired.fastq.gz,NT-BG293LV06-2Bmerged_S10_L001_R1_001.filtered.trimmed.paired.fastq.gz	ENCORE2	29826
WDR6-BG293LV06-89B_S41_L001_R2_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set72/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3083	NovaSeq6000	100	paired-ended	2				NT-BG293LV06-1Bmerged_S9_L001_R2_001.filtered.trimmed.paired.fastq.gz,NT-BG293LV06-2Bmerged_S10_L001_R2_001.filtered.trimmed.paired.fastq.gz	ENCORE2	29898
WDR6-BG293LV06-89B_Aligned.sortedByCoord.out.bam	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bam	alignments	knockdown followed by RNA-seq	Graveley Lab	3083					GRCh38	V40	WDR6-BG293LV06-89B_S41_L001_R1_001.filtered.trimmed.paired.fastq.gz,WDR6-BG293LV06-89B_S41_L001_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	29970
WDR6-BG293LV06-89B_Signal.UniqueMultiple.strand-.bw	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bigWig-all	minus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3083					GRCh38	V40	WDR6-BG293LV06-89B_Aligned.sortedByCoord.out.bam		ENCORE2	30042
WDR6-BG293LV06-89B_Signal.UniqueMultiple.strand+.bw	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bigWig+all	plus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3083					GRCh38	V40	WDR6-BG293LV06-89B_Aligned.sortedByCoord.out.bam		ENCORE2	30114
WDR6-BG293LV06-89B_Signal.Unique.strand-.bw	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bigWig-unique	minus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3083					GRCh38	V40	WDR6-BG293LV06-89B_Aligned.sortedByCoord.out.bam		ENCORE2	30186
WDR6-BG293LV06-89B_Signal.Unique.strand+.bw	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bigWig+unique	plus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3083					GRCh38	V40	WDR6-BG293LV06-89B_Aligned.sortedByCoord.out.bam		ENCORE2	30258
WDR6-BG293LV06-89B_quant.sf	ENCODE_DATA/set72/salmon_ENCORE2_file_links	transcript_quantification	transcript quantifications	knockdown followed by RNA-seq	Graveley Lab	3083					GRCh38	V40	WDR6-BG293LV06-89B_S41_L001_R1_001.filtered.trimmed.paired.fastq.gz,WDR6-BG293LV06-89B_S41_L001_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	30330
WDR6-BG293LV06-90B_S42_L001_R1_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set72/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3084	NovaSeq6000	100	paired-ended	1				NT-BG293LV06-1Bmerged_S9_L001_R1_001.filtered.trimmed.paired.fastq.gz,NT-BG293LV06-2Bmerged_S10_L001_R1_001.filtered.trimmed.paired.fastq.gz	ENCORE2	29827
WDR6-BG293LV06-90B_S42_L001_R2_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set72/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3084	NovaSeq6000	100	paired-ended	2				NT-BG293LV06-1Bmerged_S9_L001_R2_001.filtered.trimmed.paired.fastq.gz,NT-BG293LV06-2Bmerged_S10_L001_R2_001.filtered.trimmed.paired.fastq.gz	ENCORE2	29899
WDR6-BG293LV06-90B_Aligned.sortedByCoord.out.bam	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bam	alignments	knockdown followed by RNA-seq	Graveley Lab	3084					GRCh38	V40	WDR6-BG293LV06-90B_S42_L001_R1_001.filtered.trimmed.paired.fastq.gz,WDR6-BG293LV06-90B_S42_L001_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	29971
WDR6-BG293LV06-90B_Signal.UniqueMultiple.strand-.bw	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bigWig-all	minus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3084					GRCh38	V40	WDR6-BG293LV06-90B_Aligned.sortedByCoord.out.bam		ENCORE2	30043
WDR6-BG293LV06-90B_Signal.UniqueMultiple.strand+.bw	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bigWig+all	plus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3084					GRCh38	V40	WDR6-BG293LV06-90B_Aligned.sortedByCoord.out.bam		ENCORE2	30115
WDR6-BG293LV06-90B_Signal.Unique.strand-.bw	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bigWig-unique	minus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3084					GRCh38	V40	WDR6-BG293LV06-90B_Aligned.sortedByCoord.out.bam		ENCORE2	30187
WDR6-BG293LV06-90B_Signal.Unique.strand+.bw	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bigWig+unique	plus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3084					GRCh38	V40	WDR6-BG293LV06-90B_Aligned.sortedByCoord.out.bam		ENCORE2	30259
WDR6-BG293LV06-90B_quant.sf	ENCODE_DATA/set72/salmon_ENCORE2_file_links	transcript_quantification	transcript quantifications	knockdown followed by RNA-seq	Graveley Lab	3084					GRCh38	V40	WDR6-BG293LV06-90B_S42_L001_R1_001.filtered.trimmed.paired.fastq.gz,WDR6-BG293LV06-90B_S42_L001_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	30331