RPS19BP1 RNA-Seq in 293T

Experiment Information (Status: Released)

	BG293LV06-87	BG293LV06-88
idx	0	0
TRCN#_or_BGC#	TRCN0000172981	TRCN0000172981
shRNA_or_gRNA_sequence	CCGGGCCAAGACCAAGAAGAAGAAGCTCGAGCTTCTTCTTCTTGGTCTTGGCTTTTTTG	CCGGGCCAAGACCAAGAAGAAGAAGCTCGAGCTTCTTCTTCTTGGTCTTGGCTTTTTTG
PAM
Name	RPS19BP1	RPS19BP1
Sample_ID	BG293LV06-87	BG293LV06-88
transduction_Date	9/5/24	9/5/24
days	D4	D4
RBP_name	RPS19BP1	RPS19BP1
qPCR_result	95.0	95.2
Ave_qPCR	95.1
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc	0	0
WB_result
Ave_WB
WB_DONE_date
MW
IP
antibody_Cat#
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM	0	0
Rep2_TPM	0	0
Action	Ready	Ready
Library_start_date
repeat_library
Note
ID	12222	12223

Western Blot Western Blot info was not avaliable

Experiment Status

	BG293LV06-87	BG293LV06-88
Sample_ID	BG293LV06-87	BG293LV06-88
Sample Name
Sample Name	RPS19BP1-BG293LV06-87B	RPS19BP1-BG293LV06-88B
RBP	RPS19BP1	RPS19BP1
Cell_Line	293T	293T
Exp UID
Status	Released	Released
Status_date	2025-04-21	2025-04-21
Project	ENCORE2	ENCORE2
Note
ID	13524	13525

Library-Prep
Sequencing

Library-Prep Information

	BG293LV06-87	BG293LV06-88
Sample #	87	88
Sample Name	RPS19BP1-BG293LV06-87B	RPS19BP1-BG293LV06-88B
Sample_Name_Alias
Index Well Position	G04	H04
Index_table	IDT_UniqueDualIndex_96_V2	IDT_UniqueDualIndex_96_V2
LibPrep_date	2024-10-02	2024-10-02
Lib_ID	Lib-241002	Lib-241002
Tecan_Location
Tecan
Tecan_date
Size_bp	272	273
Peak_Molarity	87.70	65.10
libSampleQC_DNA_Well	DNA_Library_set72/set1/G4.png	DNA_Library_set72/set1/H4.png
RIN	9.8	9.7
libSample_RNA_Well	RNA_Library_set72/set1/G4.png	RNA_Library_set72/set1/H4.png
SampleQC_method	TapeStation_2022	TapeStation_2022
SampleQC_date	2024-10-04	2024-10-04
Sample_ID	BG293LV06-87	BG293LV06-88
RBP	RPS19BP1	RPS19BP1
Batch_ID	BG293LV06	BG293LV06
WB_result	0.000	0.000
Library Description	TruSeq mRNA	TruSeq mRNA
Repeat_Library_Suffix	B	B
Lib_Status	SendToSequence	SendToSequence
Project	ENCORE2	ENCORE2
ID	4512	4513

Sequencing Information

	BG293LV06-87	BG293LV06-88
Sample_ID	BG293LV06-87	BG293LV06-88
Sample Name	RPS19BP1-BG293LV06-87B	RPS19BP1-BG293LV06-88B
Pool ID	Pool-241004	Pool-241004
LocalServer_folder	set72	set72
total_reads	25,781,619	37,366,336
total_aligned_reads	24,131,396	33,860,996
unique_aligned_reads	22,498,907	31,742,437
percent_uniqueAligned	0.87267	0.84949
correlation_replicates	0.992955	0.992955
spikein_reads	79,682	85,786
percent_spikeins	0.00309	0.00230
original_ReadLength	101	101
QC_Status	Submitted	Submitted
ID	3081	3082

Data Submission Information DCC submission information was not avaliable

File Information

file_name	file_path	file_format	output_type	assay_type	lab	Sample ID	platform	read_length	run_type	paired_end	assembly	genome_annotation	derived_from	controlled_by	Project	FILE_ID
RPS19BP1-BG293LV06-87B_S39_L001_R1_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set72/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3081	NovaSeq6000	100	paired-ended	1				NT-BG293LV06-1Bmerged_S9_L001_R1_001.filtered.trimmed.paired.fastq.gz,NT-BG293LV06-2Bmerged_S10_L001_R1_001.filtered.trimmed.paired.fastq.gz	ENCORE2	29810
RPS19BP1-BG293LV06-87B_S39_L001_R2_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set72/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3081	NovaSeq6000	100	paired-ended	2				NT-BG293LV06-1Bmerged_S9_L001_R2_001.filtered.trimmed.paired.fastq.gz,NT-BG293LV06-2Bmerged_S10_L001_R2_001.filtered.trimmed.paired.fastq.gz	ENCORE2	29882
RPS19BP1-BG293LV06-87B_Aligned.sortedByCoord.out.bam	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bam	alignments	knockdown followed by RNA-seq	Graveley Lab	3081					GRCh38	V40	RPS19BP1-BG293LV06-87B_S39_L001_R1_001.filtered.trimmed.paired.fastq.gz,RPS19BP1-BG293LV06-87B_S39_L001_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	29954
RPS19BP1-BG293LV06-87B_Signal.UniqueMultiple.strand-.bw	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bigWig-all	minus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3081					GRCh38	V40	RPS19BP1-BG293LV06-87B_Aligned.sortedByCoord.out.bam		ENCORE2	30026
RPS19BP1-BG293LV06-87B_Signal.UniqueMultiple.strand+.bw	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bigWig+all	plus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3081					GRCh38	V40	RPS19BP1-BG293LV06-87B_Aligned.sortedByCoord.out.bam		ENCORE2	30098
RPS19BP1-BG293LV06-87B_Signal.Unique.strand-.bw	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bigWig-unique	minus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3081					GRCh38	V40	RPS19BP1-BG293LV06-87B_Aligned.sortedByCoord.out.bam		ENCORE2	30170
RPS19BP1-BG293LV06-87B_Signal.Unique.strand+.bw	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bigWig+unique	plus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3081					GRCh38	V40	RPS19BP1-BG293LV06-87B_Aligned.sortedByCoord.out.bam		ENCORE2	30242
RPS19BP1-BG293LV06-87B_quant.sf	ENCODE_DATA/set72/salmon_ENCORE2_file_links	transcript_quantification	transcript quantifications	knockdown followed by RNA-seq	Graveley Lab	3081					GRCh38	V40	RPS19BP1-BG293LV06-87B_S39_L001_R1_001.filtered.trimmed.paired.fastq.gz,RPS19BP1-BG293LV06-87B_S39_L001_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	30314
RPS19BP1-BG293LV06-88B_S40_L001_R1_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set72/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3082	NovaSeq6000	100	paired-ended	1				NT-BG293LV06-1Bmerged_S9_L001_R1_001.filtered.trimmed.paired.fastq.gz,NT-BG293LV06-2Bmerged_S10_L001_R1_001.filtered.trimmed.paired.fastq.gz	ENCORE2	29811
RPS19BP1-BG293LV06-88B_S40_L001_R2_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set72/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3082	NovaSeq6000	100	paired-ended	2				NT-BG293LV06-1Bmerged_S9_L001_R2_001.filtered.trimmed.paired.fastq.gz,NT-BG293LV06-2Bmerged_S10_L001_R2_001.filtered.trimmed.paired.fastq.gz	ENCORE2	29883
RPS19BP1-BG293LV06-88B_Aligned.sortedByCoord.out.bam	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bam	alignments	knockdown followed by RNA-seq	Graveley Lab	3082					GRCh38	V40	RPS19BP1-BG293LV06-88B_S40_L001_R1_001.filtered.trimmed.paired.fastq.gz,RPS19BP1-BG293LV06-88B_S40_L001_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	29955
RPS19BP1-BG293LV06-88B_Signal.UniqueMultiple.strand-.bw	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bigWig-all	minus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3082					GRCh38	V40	RPS19BP1-BG293LV06-88B_Aligned.sortedByCoord.out.bam		ENCORE2	30027
RPS19BP1-BG293LV06-88B_Signal.UniqueMultiple.strand+.bw	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bigWig+all	plus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3082					GRCh38	V40	RPS19BP1-BG293LV06-88B_Aligned.sortedByCoord.out.bam		ENCORE2	30099
RPS19BP1-BG293LV06-88B_Signal.Unique.strand-.bw	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bigWig-unique	minus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3082					GRCh38	V40	RPS19BP1-BG293LV06-88B_Aligned.sortedByCoord.out.bam		ENCORE2	30171
RPS19BP1-BG293LV06-88B_Signal.Unique.strand+.bw	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bigWig+unique	plus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3082					GRCh38	V40	RPS19BP1-BG293LV06-88B_Aligned.sortedByCoord.out.bam		ENCORE2	30243
RPS19BP1-BG293LV06-88B_quant.sf	ENCODE_DATA/set72/salmon_ENCORE2_file_links	transcript_quantification	transcript quantifications	knockdown followed by RNA-seq	Graveley Lab	3082					GRCh38	V40	RPS19BP1-BG293LV06-88B_S40_L001_R1_001.filtered.trimmed.paired.fastq.gz,RPS19BP1-BG293LV06-88B_S40_L001_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	30315