HNRNPH2 RNA-Seq in 293T

Experiment Information (Status: Released)

	BG293LV06-71	BG293LV06-72
idx	0	0
TRCN#_or_BGC#	TRCN0000075093	TRCN0000075093
shRNA_or_gRNA_sequence	CCGGGCTTACTGTAAAGTGGAAGTTCTCGAGAACTTCCACTTTACAGTAAGCTTTTTG	CCGGGCTTACTGTAAAGTGGAAGTTCTCGAGAACTTCCACTTTACAGTAAGCTTTTTG
PAM
Name	HNRNPH2	HNRNPH2
Sample_ID	BG293LV06-71	BG293LV06-72
transduction_Date	9/5/24	9/5/24
days	D4	D4
RBP_name	HNRNPH2	HNRNPH2
qPCR_result	85.7	84.6
Ave_qPCR	85.2
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc	0	0
WB_result
Ave_WB
WB_DONE_date
MW
IP
antibody_Cat#
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM	0	0
Rep2_TPM	0	0
Action	Ready	Ready
Library_start_date
repeat_library
Note
ID	12206	12207

Western Blot Western Blot info was not avaliable

Experiment Status

	BG293LV06-71	BG293LV06-72
Sample_ID	BG293LV06-71	BG293LV06-72
Sample Name
Sample Name	HNRNPH2-BG293LV06-71B	HNRNPH2-BG293LV06-72B
RBP	HNRNPH2	HNRNPH2
Cell_Line	293T	293T
Exp UID
Status	Released	Released
Status_date	2025-04-21	2025-04-21
Project	ENCORE2	ENCORE2
Note
ID	13508	13509

Library-Prep
Sequencing

Library-Prep Information

	BG293LV06-71	BG293LV06-72
Sample #	73	74
Sample Name	HNRNPH2-BG293LV06-71B	HNRNPH2-BG293LV06-72B
Sample_Name_Alias
Index Well Position	A03	B03
Index_table	IDT_UniqueDualIndex_96_V2	IDT_UniqueDualIndex_96_V2
LibPrep_date	2024-10-02	2024-10-02
Lib_ID	Lib-241002	Lib-241002
Tecan_Location
Tecan
Tecan_date
Size_bp	278	288
Peak_Molarity	110.00	82.80
libSampleQC_DNA_Well	DNA_Library_set72/set1/A3.png	DNA_Library_set72/set1/B3.png
RIN	9.9	9.9
libSample_RNA_Well	RNA_Library_set72/set1/A3.png	RNA_Library_set72/set1/B3.png
SampleQC_method	TapeStation_2022	TapeStation_2022
SampleQC_date	2024-10-04	2024-10-04
Sample_ID	BG293LV06-71	BG293LV06-72
RBP	HNRNPH2	HNRNPH2
Batch_ID	BG293LV06	BG293LV06
WB_result	0.000	0.000
Library Description	TruSeq mRNA	TruSeq mRNA
Repeat_Library_Suffix	B	B
Lib_Status	SendToSequence	SendToSequence
Project	ENCORE2	ENCORE2
ID	4498	4499

Sequencing Information

	BG293LV06-71	BG293LV06-72
Sample_ID	BG293LV06-71	BG293LV06-72
Sample Name	HNRNPH2-BG293LV06-71B	HNRNPH2-BG293LV06-72B
Pool ID	Pool-241004	Pool-241004
LocalServer_folder	set72	set72
total_reads	23,478,729	22,072,263
total_aligned_reads	20,812,171	19,498,513
unique_aligned_reads	19,349,939	18,139,814
percent_uniqueAligned	0.82415	0.82184
correlation_replicates	0.981052	0.981052
spikein_reads	104,362	59,586
percent_spikeins	0.00444	0.00270
original_ReadLength	101	101
QC_Status	Submitted	Submitted
ID	3067	3068

Data Submission Information DCC submission information was not avaliable

File Information

file_name	file_path	file_format	output_type	assay_type	lab	Sample ID	platform	read_length	run_type	paired_end	assembly	genome_annotation	derived_from	controlled_by	Project	FILE_ID
HNRNPH2-BG293LV06-71B_S25_L001_R1_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set72/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3067	NovaSeq6000	100	paired-ended	1				NT-BG293LV06-1Bmerged_S9_L001_R1_001.filtered.trimmed.paired.fastq.gz,NT-BG293LV06-2Bmerged_S10_L001_R1_001.filtered.trimmed.paired.fastq.gz	ENCORE2	29784
HNRNPH2-BG293LV06-71B_S25_L001_R2_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set72/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3067	NovaSeq6000	100	paired-ended	2				NT-BG293LV06-1Bmerged_S9_L001_R2_001.filtered.trimmed.paired.fastq.gz,NT-BG293LV06-2Bmerged_S10_L001_R2_001.filtered.trimmed.paired.fastq.gz	ENCORE2	29856
HNRNPH2-BG293LV06-71B_Aligned.sortedByCoord.out.bam	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bam	alignments	knockdown followed by RNA-seq	Graveley Lab	3067					GRCh38	V40	HNRNPH2-BG293LV06-71B_S25_L001_R1_001.filtered.trimmed.paired.fastq.gz,HNRNPH2-BG293LV06-71B_S25_L001_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	29928
HNRNPH2-BG293LV06-71B_Signal.UniqueMultiple.strand-.bw	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bigWig-all	minus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3067					GRCh38	V40	HNRNPH2-BG293LV06-71B_Aligned.sortedByCoord.out.bam		ENCORE2	30000
HNRNPH2-BG293LV06-71B_Signal.UniqueMultiple.strand+.bw	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bigWig+all	plus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3067					GRCh38	V40	HNRNPH2-BG293LV06-71B_Aligned.sortedByCoord.out.bam		ENCORE2	30072
HNRNPH2-BG293LV06-71B_Signal.Unique.strand-.bw	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bigWig-unique	minus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3067					GRCh38	V40	HNRNPH2-BG293LV06-71B_Aligned.sortedByCoord.out.bam		ENCORE2	30144
HNRNPH2-BG293LV06-71B_Signal.Unique.strand+.bw	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bigWig+unique	plus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3067					GRCh38	V40	HNRNPH2-BG293LV06-71B_Aligned.sortedByCoord.out.bam		ENCORE2	30216
HNRNPH2-BG293LV06-71B_quant.sf	ENCODE_DATA/set72/salmon_ENCORE2_file_links	transcript_quantification	transcript quantifications	knockdown followed by RNA-seq	Graveley Lab	3067					GRCh38	V40	HNRNPH2-BG293LV06-71B_S25_L001_R1_001.filtered.trimmed.paired.fastq.gz,HNRNPH2-BG293LV06-71B_S25_L001_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	30288
HNRNPH2-BG293LV06-72B_S26_L001_R1_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set72/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3068	NovaSeq6000	100	paired-ended	1				NT-BG293LV06-1Bmerged_S9_L001_R1_001.filtered.trimmed.paired.fastq.gz,NT-BG293LV06-2Bmerged_S10_L001_R1_001.filtered.trimmed.paired.fastq.gz	ENCORE2	29785
HNRNPH2-BG293LV06-72B_S26_L001_R2_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set72/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3068	NovaSeq6000	100	paired-ended	2				NT-BG293LV06-1Bmerged_S9_L001_R2_001.filtered.trimmed.paired.fastq.gz,NT-BG293LV06-2Bmerged_S10_L001_R2_001.filtered.trimmed.paired.fastq.gz	ENCORE2	29857
HNRNPH2-BG293LV06-72B_Aligned.sortedByCoord.out.bam	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bam	alignments	knockdown followed by RNA-seq	Graveley Lab	3068					GRCh38	V40	HNRNPH2-BG293LV06-72B_S26_L001_R1_001.filtered.trimmed.paired.fastq.gz,HNRNPH2-BG293LV06-72B_S26_L001_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	29929
HNRNPH2-BG293LV06-72B_Signal.UniqueMultiple.strand-.bw	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bigWig-all	minus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3068					GRCh38	V40	HNRNPH2-BG293LV06-72B_Aligned.sortedByCoord.out.bam		ENCORE2	30001
HNRNPH2-BG293LV06-72B_Signal.UniqueMultiple.strand+.bw	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bigWig+all	plus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3068					GRCh38	V40	HNRNPH2-BG293LV06-72B_Aligned.sortedByCoord.out.bam		ENCORE2	30073
HNRNPH2-BG293LV06-72B_Signal.Unique.strand-.bw	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bigWig-unique	minus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3068					GRCh38	V40	HNRNPH2-BG293LV06-72B_Aligned.sortedByCoord.out.bam		ENCORE2	30145
HNRNPH2-BG293LV06-72B_Signal.Unique.strand+.bw	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bigWig+unique	plus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3068					GRCh38	V40	HNRNPH2-BG293LV06-72B_Aligned.sortedByCoord.out.bam		ENCORE2	30217
HNRNPH2-BG293LV06-72B_quant.sf	ENCODE_DATA/set72/salmon_ENCORE2_file_links	transcript_quantification	transcript quantifications	knockdown followed by RNA-seq	Graveley Lab	3068					GRCh38	V40	HNRNPH2-BG293LV06-72B_S26_L001_R1_001.filtered.trimmed.paired.fastq.gz,HNRNPH2-BG293LV06-72B_S26_L001_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	30289