RBM11 RNA-Seq in 293T

Experiment Information (Status: Released)

	BG293LV06-45	BG293LV06-46
idx	0	0
TRCN#_or_BGC#	TRCN0000162312	TRCN0000162312
shRNA_or_gRNA_sequence	CCGGCCCAGCTCATATAAATGGACTCTCGAGAGTCCATTTATATGAGCTGGGTTTTTTG	CCGGCCCAGCTCATATAAATGGACTCTCGAGAGTCCATTTATATGAGCTGGGTTTTTTG
PAM
Name	RBM11	RBM11
Sample_ID	BG293LV06-45	BG293LV06-46
transduction_Date	9/5/24	9/5/24
days	D4	D4
RBP_name	RBM11	RBM11
qPCR_result	58.3	48.7
Ave_qPCR	53.5
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc	0	0
WB_result
Ave_WB
WB_DONE_date
MW
IP
antibody_Cat#
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM	0	0
Rep2_TPM	0	0
Action	Ready	Ready
Library_start_date
repeat_library
Note
ID	12180	12181

Western Blot Western Blot info was not avaliable

Experiment Status

	BG293LV06-45	BG293LV06-46
Sample_ID	BG293LV06-45	BG293LV06-46
Sample Name
Sample Name	RBM11-BG293LV06-45	RBM11-BG293LV06-46
RBP	RBM11	RBM11
Cell_Line	293T	293T
Exp UID
Status	Released	Released
Status_date	2025-04-21	2025-04-21
Project	ENCORE2	ENCORE2
Note
ID	13482	13483

Library-Prep
Sequencing

Library-Prep Information

	BG293LV06-45	BG293LV06-46
Sample #	47	48
Sample Name	RBM11-BG293LV06-45	RBM11-BG293LV06-46
Sample_Name_Alias
Index Well Position	G06	H06
Index_table	IDT_UniqueDualIndex_96_V2	IDT_UniqueDualIndex_96_V2
LibPrep_date	2024-09-25	2024-09-25
Lib_ID	Lib-240925B	Lib-240925B
Tecan_Location
Tecan
Tecan_date
Size_bp	276	278
Peak_Molarity	88.30	66.10
libSampleQC_DNA_Well	DNA_Library_set71/set1/G6.png	DNA_Library_set71/set1/H6.png
RIN	10.0	10.0
libSample_RNA_Well	RNA_Library_set71/set1/G6.png	RNA_Library_set71/set1/H6.png
SampleQC_method	TapeStation_2022	TapeStation_2022
SampleQC_date	2024-09-30	2024-09-30
Sample_ID	BG293LV06-45	BG293LV06-46
RBP	RBM11	RBM11
Batch_ID	BG293LV06	BG293LV06
WB_result	0.000	0.000
Library Description	TruSeq mRNA	TruSeq mRNA
Repeat_Library_Suffix
Lib_Status	SendToSequence	SendToSequence
Project	ENCORE2	ENCORE2
ID	4472	4473

Sequencing Information

	BG293LV06-45	BG293LV06-46
Sample_ID	BG293LV06-45	BG293LV06-46
Sample Name	RBM11-BG293LV06-45	RBM11-BG293LV06-46
Pool ID	Pool-240930	Pool-240930
LocalServer_folder	set71	set71
total_reads	41,444,201	36,434,660
total_aligned_reads	38,279,222	33,456,321
unique_aligned_reads	35,793,616	31,293,967
percent_uniqueAligned	0.86366	0.85891
correlation_replicates	0.995960	0.995960
spikein_reads	84,210	109,972
percent_spikeins	0.00203	0.00302
original_ReadLength	101	101
QC_Status	Submitted	Submitted
ID	3041	3042

Data Submission Information DCC submission information was not avaliable

File Information

file_name	file_path	file_format	output_type	assay_type	lab	Sample ID	platform	read_length	run_type	paired_end	assembly	genome_annotation	derived_from	controlled_by	Project	FILE_ID
RBM11-BG293LV06-45_S47_L002_R1_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set71/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3041	NovaSeq6000	100	paired-ended	1				NT-BG293LV06-1merged_S1_L002_R1_001.filtered.trimmed.paired.fastq.gz,NT-BG293LV06-2merged_S2_L002_R1_001.filtered.trimmed.paired.fastq.gz	ENCORE2	29359
RBM11-BG293LV06-45_S47_L002_R2_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set71/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3041	NovaSeq6000	100	paired-ended	2				NT-BG293LV06-1merged_S1_L002_R2_001.filtered.trimmed.paired.fastq.gz,NT-BG293LV06-2merged_S2_L002_R2_001.filtered.trimmed.paired.fastq.gz	ENCORE2	29423
RBM11-BG293LV06-45_Aligned.sortedByCoord.out.bam	ENCODE_DATA/set71/STAR_ENCORE2_trimmed_file_links	bam	alignments	knockdown followed by RNA-seq	Graveley Lab	3041					GRCh38	V40	RBM11-BG293LV06-45_S47_L002_R1_001.filtered.trimmed.paired.fastq.gz,RBM11-BG293LV06-45_S47_L002_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	29488
RBM11-BG293LV06-45_Signal.UniqueMultiple.strand-.bw	ENCODE_DATA/set71/STAR_ENCORE2_trimmed_file_links	bigWig-all	minus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3041					GRCh38	V40	RBM11-BG293LV06-45_Aligned.sortedByCoord.out.bam		ENCORE2	29552
RBM11-BG293LV06-45_Signal.UniqueMultiple.strand+.bw	ENCODE_DATA/set71/STAR_ENCORE2_trimmed_file_links	bigWig+all	plus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3041					GRCh38	V40	RBM11-BG293LV06-45_Aligned.sortedByCoord.out.bam		ENCORE2	29616
RBM11-BG293LV06-45_Signal.Unique.strand-.bw	ENCODE_DATA/set71/STAR_ENCORE2_trimmed_file_links	bigWig-unique	minus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3041					GRCh38	V40	RBM11-BG293LV06-45_Aligned.sortedByCoord.out.bam		ENCORE2	29680
RBM11-BG293LV06-45_quant.sf	ENCODE_DATA/set71/salmon_ENCORE2_file_links	transcript_quantification	transcript quantifications	knockdown followed by RNA-seq	Graveley Lab	3041					GRCh38	V40	RBM11-BG293LV06-45_S47_L002_R1_001.filtered.trimmed.paired.fastq.gz,RBM11-BG293LV06-45_S47_L002_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	29744
RBM11-BG293LV06-46_S48_L002_R1_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set71/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3042	NovaSeq6000	100	paired-ended	1				NT-BG293LV06-1merged_S1_L002_R1_001.filtered.trimmed.paired.fastq.gz,NT-BG293LV06-2merged_S2_L002_R1_001.filtered.trimmed.paired.fastq.gz	ENCORE2	29360
RBM11-BG293LV06-46_S48_L002_R2_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set71/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3042	NovaSeq6000	100	paired-ended	2				NT-BG293LV06-1merged_S1_L002_R2_001.filtered.trimmed.paired.fastq.gz,NT-BG293LV06-2merged_S2_L002_R2_001.filtered.trimmed.paired.fastq.gz	ENCORE2	29424
RBM11-BG293LV06-46_Aligned.sortedByCoord.out.bam	ENCODE_DATA/set71/STAR_ENCORE2_trimmed_file_links	bam	alignments	knockdown followed by RNA-seq	Graveley Lab	3042					GRCh38	V40	RBM11-BG293LV06-46_S48_L002_R1_001.filtered.trimmed.paired.fastq.gz,RBM11-BG293LV06-46_S48_L002_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	29489
RBM11-BG293LV06-46_Signal.UniqueMultiple.strand-.bw	ENCODE_DATA/set71/STAR_ENCORE2_trimmed_file_links	bigWig-all	minus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3042					GRCh38	V40	RBM11-BG293LV06-46_Aligned.sortedByCoord.out.bam		ENCORE2	29553
RBM11-BG293LV06-46_Signal.UniqueMultiple.strand+.bw	ENCODE_DATA/set71/STAR_ENCORE2_trimmed_file_links	bigWig+all	plus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3042					GRCh38	V40	RBM11-BG293LV06-46_Aligned.sortedByCoord.out.bam		ENCORE2	29617
RBM11-BG293LV06-46_Signal.Unique.strand-.bw	ENCODE_DATA/set71/STAR_ENCORE2_trimmed_file_links	bigWig-unique	minus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3042					GRCh38	V40	RBM11-BG293LV06-46_Aligned.sortedByCoord.out.bam		ENCORE2	29681
RBM11-BG293LV06-46_quant.sf	ENCODE_DATA/set71/salmon_ENCORE2_file_links	transcript_quantification	transcript quantifications	knockdown followed by RNA-seq	Graveley Lab	3042					GRCh38	V40	RBM11-BG293LV06-46_S48_L002_R1_001.filtered.trimmed.paired.fastq.gz,RBM11-BG293LV06-46_S48_L002_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	29745