RBM10 RNA-Seq in 293T

Experiment Information (Status: Released)

	BG293LV06-19	BG293LV06-20
idx	0	0
TRCN#_or_BGC#	TRCN0000233278	TRCN0000233278
shRNA_or_gRNA_sequence	CCGGTCCAACGTGCGCGTCATAAAGCTCGAGCTTTATGACGCGCACGTTGGATTTTTG	CCGGTCCAACGTGCGCGTCATAAAGCTCGAGCTTTATGACGCGCACGTTGGATTTTTG
PAM
Name	RBM10	RBM10
Sample_ID	BG293LV06-19	BG293LV06-20
transduction_Date	9/5/24	9/5/24
days	D4	D4
RBP_name	RBM10	RBM10
qPCR_result	50.7	60.0
Ave_qPCR	55.4
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc	0	0
WB_result
Ave_WB
WB_DONE_date
MW
IP
antibody_Cat#
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM	0	0
Rep2_TPM	0	0
Action	Ready	Ready
Library_start_date
repeat_library
Note
ID	12154	12155

Western Blot Western Blot info was not avaliable

Experiment Status

	BG293LV06-19	BG293LV06-20
Sample_ID	BG293LV06-19	BG293LV06-20
Sample Name
Sample Name	RBM10-BG293LV06-19	RBM10-BG293LV06-20
RBP	RBM10	RBM10
Cell_Line	293T	293T
Exp UID
Status	Released	Released
Status_date	2025-04-21	2025-04-21
Project	ENCORE2	ENCORE2
Note
ID	13456	13457

Library-Prep
Sequencing

Library-Prep Information

	BG293LV06-19	BG293LV06-20
Sample #	23	24
Sample Name	RBM10-BG293LV06-19	RBM10-BG293LV06-20
Sample_Name_Alias
Index Well Position	G03	H03
Index_table	IDT_UniqueDualIndex_96_V2	IDT_UniqueDualIndex_96_V2
LibPrep_date	2024-09-25	2024-09-25
Lib_ID	Lib-240925B	Lib-240925B
Tecan_Location
Tecan
Tecan_date
Size_bp	275	269
Peak_Molarity	77.90	70.40
libSampleQC_DNA_Well	DNA_Library_set71/set1/G3.png	DNA_Library_set71/set1/H3.png
RIN	9.9	9.9
libSample_RNA_Well	RNA_Library_set71/set1/G3.png	RNA_Library_set71/set1/H3.png
SampleQC_method	TapeStation_2022	TapeStation_2022
SampleQC_date	2024-09-30	2024-09-30
Sample_ID	BG293LV06-19	BG293LV06-20
RBP	RBM10	RBM10
Batch_ID	BG293LV06	BG293LV06
WB_result	0.000	0.000
Library Description	TruSeq mRNA	TruSeq mRNA
Repeat_Library_Suffix
Lib_Status	SendToSequence	SendToSequence
Project	ENCORE2	ENCORE2
ID	4448	4449

Sequencing Information

	BG293LV06-19	BG293LV06-20
Sample_ID	BG293LV06-19	BG293LV06-20
Sample Name	RBM10-BG293LV06-19	RBM10-BG293LV06-20
Pool ID	Pool-240930	Pool-240930
LocalServer_folder	set71	set71
total_reads	48,134,527	25,193,586
total_aligned_reads	43,603,708	20,539,687
unique_aligned_reads	40,740,866	19,194,621
percent_uniqueAligned	0.84640	0.76189
correlation_replicates	0.992130	0.992130
spikein_reads	122,260	78,094
percent_spikeins	0.00254	0.00310
original_ReadLength	101	101
QC_Status	Submitted	Submitted
ID	3017	3018

Data Submission Information DCC submission information was not avaliable

File Information

file_name	file_path	file_format	output_type	assay_type	lab	Sample ID	platform	read_length	run_type	paired_end	assembly	genome_annotation	derived_from	controlled_by	Project	FILE_ID
RBM10-BG293LV06-19_S23_L002_R1_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set71/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3017	NovaSeq6000	100	paired-ended	1				NT-BG293LV06-1merged_S1_L002_R1_001.filtered.trimmed.paired.fastq.gz,NT-BG293LV06-2merged_S2_L002_R1_001.filtered.trimmed.paired.fastq.gz	ENCORE2	29357
RBM10-BG293LV06-19_S23_L002_R2_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set71/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3017	NovaSeq6000	100	paired-ended	2				NT-BG293LV06-1merged_S1_L002_R2_001.filtered.trimmed.paired.fastq.gz,NT-BG293LV06-2merged_S2_L002_R2_001.filtered.trimmed.paired.fastq.gz	ENCORE2	29421
RBM10-BG293LV06-19_Aligned.sortedByCoord.out.bam	ENCODE_DATA/set71/STAR_ENCORE2_trimmed_file_links	bam	alignments	knockdown followed by RNA-seq	Graveley Lab	3017					GRCh38	V40	RBM10-BG293LV06-19_S23_L002_R1_001.filtered.trimmed.paired.fastq.gz,RBM10-BG293LV06-19_S23_L002_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	29486
RBM10-BG293LV06-19_Signal.UniqueMultiple.strand-.bw	ENCODE_DATA/set71/STAR_ENCORE2_trimmed_file_links	bigWig-all	minus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3017					GRCh38	V40	RBM10-BG293LV06-19_Aligned.sortedByCoord.out.bam		ENCORE2	29550
RBM10-BG293LV06-19_Signal.UniqueMultiple.strand+.bw	ENCODE_DATA/set71/STAR_ENCORE2_trimmed_file_links	bigWig+all	plus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3017					GRCh38	V40	RBM10-BG293LV06-19_Aligned.sortedByCoord.out.bam		ENCORE2	29614
RBM10-BG293LV06-19_Signal.Unique.strand-.bw	ENCODE_DATA/set71/STAR_ENCORE2_trimmed_file_links	bigWig-unique	minus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3017					GRCh38	V40	RBM10-BG293LV06-19_Aligned.sortedByCoord.out.bam		ENCORE2	29678
RBM10-BG293LV06-19_quant.sf	ENCODE_DATA/set71/salmon_ENCORE2_file_links	transcript_quantification	transcript quantifications	knockdown followed by RNA-seq	Graveley Lab	3017					GRCh38	V40	RBM10-BG293LV06-19_S23_L002_R1_001.filtered.trimmed.paired.fastq.gz,RBM10-BG293LV06-19_S23_L002_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	29742
RBM10-BG293LV06-20_S24_L002_R1_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set71/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3018	NovaSeq6000	100	paired-ended	1				NT-BG293LV06-1merged_S1_L002_R1_001.filtered.trimmed.paired.fastq.gz,NT-BG293LV06-2merged_S2_L002_R1_001.filtered.trimmed.paired.fastq.gz	ENCORE2	29358
RBM10-BG293LV06-20_S24_L002_R2_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set71/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3018	NovaSeq6000	100	paired-ended	2				NT-BG293LV06-1merged_S1_L002_R2_001.filtered.trimmed.paired.fastq.gz,NT-BG293LV06-2merged_S2_L002_R2_001.filtered.trimmed.paired.fastq.gz	ENCORE2	29422
RBM10-BG293LV06-20_Aligned.sortedByCoord.out.bam	ENCODE_DATA/set71/STAR_ENCORE2_trimmed_file_links	bam	alignments	knockdown followed by RNA-seq	Graveley Lab	3018					GRCh38	V40	RBM10-BG293LV06-20_S24_L002_R1_001.filtered.trimmed.paired.fastq.gz,RBM10-BG293LV06-20_S24_L002_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	29487
RBM10-BG293LV06-20_Signal.UniqueMultiple.strand-.bw	ENCODE_DATA/set71/STAR_ENCORE2_trimmed_file_links	bigWig-all	minus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3018					GRCh38	V40	RBM10-BG293LV06-20_Aligned.sortedByCoord.out.bam		ENCORE2	29551
RBM10-BG293LV06-20_Signal.UniqueMultiple.strand+.bw	ENCODE_DATA/set71/STAR_ENCORE2_trimmed_file_links	bigWig+all	plus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3018					GRCh38	V40	RBM10-BG293LV06-20_Aligned.sortedByCoord.out.bam		ENCORE2	29615
RBM10-BG293LV06-20_Signal.Unique.strand-.bw	ENCODE_DATA/set71/STAR_ENCORE2_trimmed_file_links	bigWig-unique	minus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3018					GRCh38	V40	RBM10-BG293LV06-20_Aligned.sortedByCoord.out.bam		ENCORE2	29679
RBM10-BG293LV06-20_quant.sf	ENCODE_DATA/set71/salmon_ENCORE2_file_links	transcript_quantification	transcript quantifications	knockdown followed by RNA-seq	Graveley Lab	3018					GRCh38	V40	RBM10-BG293LV06-20_S24_L002_R1_001.filtered.trimmed.paired.fastq.gz,RBM10-BG293LV06-20_S24_L002_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	29743