MBNL2 RNA-Seq in 293T

Experiment Information (Status: Released)

	BG293LV06-13	BG293LV06-14
idx	0	0
TRCN#_or_BGC#	TRCN0000180132	TRCN0000180132
shRNA_or_gRNA_sequence	CCGGCGGCTATTAGCTTTGCTCCTTCTCGAGAAGGAGCAAAGCTAATAGCCGTTTTTTG	CCGGCGGCTATTAGCTTTGCTCCTTCTCGAGAAGGAGCAAAGCTAATAGCCGTTTTTTG
PAM
Name	MBNL2	MBNL2
Sample_ID	BG293LV06-13	BG293LV06-14
transduction_Date	9/5/24	9/5/24
days	D4	D4
RBP_name	MBNL2	MBNL2
qPCR_result	92.6	93.0
Ave_qPCR	92.8
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc	0	0
WB_result
Ave_WB
WB_DONE_date
MW
IP
antibody_Cat#
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM	0	0
Rep2_TPM	0	0
Action	Ready	Ready
Library_start_date
repeat_library
Note
ID	12148	12149

Western Blot Western Blot info was not avaliable

Experiment Status

	BG293LV06-13	BG293LV06-14
Sample_ID	BG293LV06-13	BG293LV06-14
Sample Name
Sample Name	MBNL2-BG293LV06-13	MBNL2-BG293LV06-14
RBP	MBNL2	MBNL2
Cell_Line	293T	293T
Exp UID
Status	Released	Released
Status_date	2025-04-21	2025-04-21
Project	ENCORE2	ENCORE2
Note
ID	13450	13451

Library-Prep
Sequencing

Library-Prep Information

	BG293LV06-13	BG293LV06-14
Sample #	17	18
Sample Name	MBNL2-BG293LV06-13	MBNL2-BG293LV06-14
Sample_Name_Alias
Index Well Position	A03	B03
Index_table	IDT_UniqueDualIndex_96_V2	IDT_UniqueDualIndex_96_V2
LibPrep_date	2024-09-25	2024-09-25
Lib_ID	Lib-240925B	Lib-240925B
Tecan_Location
Tecan
Tecan_date
Size_bp	287	283
Peak_Molarity	77.70	78.80
libSampleQC_DNA_Well	DNA_Library_set71/set1/A3.png	DNA_Library_set71/set1/B3.png
RIN	9.9	9.9
libSample_RNA_Well	RNA_Library_set71/set1/A3.png	RNA_Library_set71/set1/B3.png
SampleQC_method	TapeStation_2022	TapeStation_2022
SampleQC_date	2024-09-30	2024-09-30
Sample_ID	BG293LV06-13	BG293LV06-14
RBP	MBNL2	MBNL2
Batch_ID	BG293LV06	BG293LV06
WB_result	0.000	0.000
Library Description	TruSeq mRNA	TruSeq mRNA
Repeat_Library_Suffix
Lib_Status	SendToSequence	SendToSequence
Project	ENCORE2	ENCORE2
ID	4442	4443

Sequencing Information

	BG293LV06-13	BG293LV06-14
Sample_ID	BG293LV06-13	BG293LV06-14
Sample Name	MBNL2-BG293LV06-13	MBNL2-BG293LV06-14
Pool ID	Pool-240930	Pool-240930
LocalServer_folder	set71	set71
total_reads	29,456,082	31,936,624
total_aligned_reads	25,942,717	28,842,684
unique_aligned_reads	24,252,455	26,968,022
percent_uniqueAligned	0.82334	0.84442
correlation_replicates	0.997185	0.997185
spikein_reads	99,956	91,064
percent_spikeins	0.00339	0.00285
original_ReadLength	101	101
QC_Status	Submitted	Submitted
ID	3011	3012

Data Submission Information DCC submission information was not avaliable

File Information

file_name	file_path	file_format	output_type	assay_type	lab	Sample ID	platform	read_length	run_type	paired_end	assembly	genome_annotation	derived_from	controlled_by	Project	FILE_ID
MBNL2-BG293LV06-13_S17_L002_R1_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set71/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3011	NovaSeq6000	100	paired-ended	1				NT-BG293LV06-1merged_S1_L002_R1_001.filtered.trimmed.paired.fastq.gz,NT-BG293LV06-2merged_S2_L002_R1_001.filtered.trimmed.paired.fastq.gz	ENCORE2	29335
MBNL2-BG293LV06-13_S17_L002_R2_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set71/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3011	NovaSeq6000	100	paired-ended	2				NT-BG293LV06-1merged_S1_L002_R2_001.filtered.trimmed.paired.fastq.gz,NT-BG293LV06-2merged_S2_L002_R2_001.filtered.trimmed.paired.fastq.gz	ENCORE2	29399
MBNL2-BG293LV06-13_Aligned.sortedByCoord.out.bam	ENCODE_DATA/set71/STAR_ENCORE2_trimmed_file_links	bam	alignments	knockdown followed by RNA-seq	Graveley Lab	3011					GRCh38	V40	MBNL2-BG293LV06-13_S17_L002_R1_001.filtered.trimmed.paired.fastq.gz,MBNL2-BG293LV06-13_S17_L002_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	29464
MBNL2-BG293LV06-13_Signal.UniqueMultiple.strand-.bw	ENCODE_DATA/set71/STAR_ENCORE2_trimmed_file_links	bigWig-all	minus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3011					GRCh38	V40	MBNL2-BG293LV06-13_Aligned.sortedByCoord.out.bam		ENCORE2	29528
MBNL2-BG293LV06-13_Signal.UniqueMultiple.strand+.bw	ENCODE_DATA/set71/STAR_ENCORE2_trimmed_file_links	bigWig+all	plus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3011					GRCh38	V40	MBNL2-BG293LV06-13_Aligned.sortedByCoord.out.bam		ENCORE2	29592
MBNL2-BG293LV06-13_Signal.Unique.strand-.bw	ENCODE_DATA/set71/STAR_ENCORE2_trimmed_file_links	bigWig-unique	minus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3011					GRCh38	V40	MBNL2-BG293LV06-13_Aligned.sortedByCoord.out.bam		ENCORE2	29656
MBNL2-BG293LV06-13_quant.sf	ENCODE_DATA/set71/salmon_ENCORE2_file_links	transcript_quantification	transcript quantifications	knockdown followed by RNA-seq	Graveley Lab	3011					GRCh38	V40	MBNL2-BG293LV06-13_S17_L002_R1_001.filtered.trimmed.paired.fastq.gz,MBNL2-BG293LV06-13_S17_L002_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	29720
MBNL2-BG293LV06-14_S18_L002_R1_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set71/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3012	NovaSeq6000	100	paired-ended	1				NT-BG293LV06-1merged_S1_L002_R1_001.filtered.trimmed.paired.fastq.gz,NT-BG293LV06-2merged_S2_L002_R1_001.filtered.trimmed.paired.fastq.gz	ENCORE2	29336
MBNL2-BG293LV06-14_S18_L002_R2_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set71/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3012	NovaSeq6000	100	paired-ended	2				NT-BG293LV06-1merged_S1_L002_R2_001.filtered.trimmed.paired.fastq.gz,NT-BG293LV06-2merged_S2_L002_R2_001.filtered.trimmed.paired.fastq.gz	ENCORE2	29400
MBNL2-BG293LV06-14_Aligned.sortedByCoord.out.bam	ENCODE_DATA/set71/STAR_ENCORE2_trimmed_file_links	bam	alignments	knockdown followed by RNA-seq	Graveley Lab	3012					GRCh38	V40	MBNL2-BG293LV06-14_S18_L002_R1_001.filtered.trimmed.paired.fastq.gz,MBNL2-BG293LV06-14_S18_L002_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	29465
MBNL2-BG293LV06-14_Signal.UniqueMultiple.strand-.bw	ENCODE_DATA/set71/STAR_ENCORE2_trimmed_file_links	bigWig-all	minus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3012					GRCh38	V40	MBNL2-BG293LV06-14_Aligned.sortedByCoord.out.bam		ENCORE2	29529
MBNL2-BG293LV06-14_Signal.UniqueMultiple.strand+.bw	ENCODE_DATA/set71/STAR_ENCORE2_trimmed_file_links	bigWig+all	plus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3012					GRCh38	V40	MBNL2-BG293LV06-14_Aligned.sortedByCoord.out.bam		ENCORE2	29593
MBNL2-BG293LV06-14_Signal.Unique.strand-.bw	ENCODE_DATA/set71/STAR_ENCORE2_trimmed_file_links	bigWig-unique	minus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3012					GRCh38	V40	MBNL2-BG293LV06-14_Aligned.sortedByCoord.out.bam		ENCORE2	29657
MBNL2-BG293LV06-14_quant.sf	ENCODE_DATA/set71/salmon_ENCORE2_file_links	transcript_quantification	transcript quantifications	knockdown followed by RNA-seq	Graveley Lab	3012					GRCh38	V40	MBNL2-BG293LV06-14_S18_L002_R1_001.filtered.trimmed.paired.fastq.gz,MBNL2-BG293LV06-14_S18_L002_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	29721