RBM22 RNA-Seq in 293T

Experiment Information (Status: Released)

	BG293LV06-11	BG293LV06-12
idx	0	0
TRCN#_or_BGC#	TRCN0000008650	TRCN0000008650
shRNA_or_gRNA_sequence	ACTGAATGTGAAATCTCGAGATTTCACATTCAGTCTGCGGN	ACTGAATGTGAAATCTCGAGATTTCACATTCAGTCTGCGGN
PAM
Name	RBM22	RBM22
Sample_ID	BG293LV06-11	BG293LV06-12
transduction_Date	9/5/24	9/5/24
days	D4	D4
RBP_name	RBM22	RBM22
qPCR_result	91.0	88.8
Ave_qPCR	89.9
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc	0	0
WB_result
Ave_WB
WB_DONE_date
MW
IP
antibody_Cat#
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM	0	0
Rep2_TPM	0	0
Action	Ready	Ready
Library_start_date
repeat_library
Note
ID	12146	12147

Western Blot Western Blot info was not avaliable

Experiment Status

	BG293LV06-11	BG293LV06-12
Sample_ID	BG293LV06-11	BG293LV06-12
Sample Name
Sample Name	RBM22-BG293LV06-11	RBM22-BG293LV06-12
RBP	RBM22	RBM22
Cell_Line	293T	293T
Exp UID
Status	Released	Released
Status_date	2025-04-21	2025-04-21
Project	ENCORE2	ENCORE2
Note
ID	13448	13449

Library-Prep
Sequencing

Library-Prep Information

	BG293LV06-11	BG293LV06-12
Sample #	15	16
Sample Name	RBM22-BG293LV06-11	RBM22-BG293LV06-12
Sample_Name_Alias
Index Well Position	G02	H02
Index_table	IDT_UniqueDualIndex_96_V2	IDT_UniqueDualIndex_96_V2
LibPrep_date	2024-09-25	2024-09-25
Lib_ID	Lib-240925B	Lib-240925B
Tecan_Location
Tecan
Tecan_date
Size_bp	284	286
Peak_Molarity	82.70	59.90
libSampleQC_DNA_Well	DNA_Library_set71/set1/G2.png	DNA_Library_set71/set1/H2.png
RIN	9.9	10.0
libSample_RNA_Well	RNA_Library_set71/set1/G2.png	RNA_Library_set71/set1/H2.png
SampleQC_method	TapeStation_2022	TapeStation_2022
SampleQC_date	2024-09-30	2024-09-30
Sample_ID	BG293LV06-11	BG293LV06-12
RBP	RBM22	RBM22
Batch_ID	BG293LV06	BG293LV06
WB_result	0.000	0.000
Library Description	TruSeq mRNA	TruSeq mRNA
Repeat_Library_Suffix
Lib_Status	SendToSequence	SendToSequence
Project	ENCORE2	ENCORE2
ID	4440	4441

Sequencing Information

	BG293LV06-11	BG293LV06-12
Sample_ID	BG293LV06-11	BG293LV06-12
Sample Name	RBM22-BG293LV06-11	RBM22-BG293LV06-12
Pool ID	Pool-240930	Pool-240930
LocalServer_folder	set71	set71
total_reads	30,350,156	49,793,185
total_aligned_reads	27,632,917	44,515,048
unique_aligned_reads	25,651,929	41,612,504
percent_uniqueAligned	0.84520	0.83571
correlation_replicates	0.968496	0.968496
spikein_reads	76,540	162,344
percent_spikeins	0.00252	0.00326
original_ReadLength	101	101
QC_Status	Submitted	Submitted
ID	3009	3010

Data Submission Information DCC submission information was not avaliable

File Information

file_name	file_path	file_format	output_type	assay_type	lab	Sample ID	platform	read_length	run_type	paired_end	assembly	genome_annotation	derived_from	controlled_by	Project	FILE_ID
RBM22-BG293LV06-11_S101_L002_R1_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set71/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3009	NovaSeq6000	100	paired-ended	1				NT-BG293LV06-1merged_S1_L002_R1_001.filtered.trimmed.paired.fastq.gz,NT-BG293LV06-2merged_S2_L002_R1_001.filtered.trimmed.paired.fastq.gz	ENCORE2	29363
RBM22-BG293LV06-11_S101_L002_R2_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set71/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3009	NovaSeq6000	100	paired-ended	2				NT-BG293LV06-1merged_S1_L002_R2_001.filtered.trimmed.paired.fastq.gz,NT-BG293LV06-2merged_S2_L002_R2_001.filtered.trimmed.paired.fastq.gz	ENCORE2	29427
RBM22-BG293LV06-11_Aligned.sortedByCoord.out.bam	ENCODE_DATA/set71/STAR_ENCORE2_trimmed_file_links	bam	alignments	knockdown followed by RNA-seq	Graveley Lab	3009					GRCh38	V40	RBM22-BG293LV06-11_S101_L002_R1_001.filtered.trimmed.paired.fastq.gz,RBM22-BG293LV06-11_S101_L002_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	29492
RBM22-BG293LV06-11_Signal.UniqueMultiple.strand-.bw	ENCODE_DATA/set71/STAR_ENCORE2_trimmed_file_links	bigWig-all	minus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3009					GRCh38	V40	RBM22-BG293LV06-11_Aligned.sortedByCoord.out.bam		ENCORE2	29556
RBM22-BG293LV06-11_Signal.UniqueMultiple.strand+.bw	ENCODE_DATA/set71/STAR_ENCORE2_trimmed_file_links	bigWig+all	plus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3009					GRCh38	V40	RBM22-BG293LV06-11_Aligned.sortedByCoord.out.bam		ENCORE2	29620
RBM22-BG293LV06-11_Signal.Unique.strand-.bw	ENCODE_DATA/set71/STAR_ENCORE2_trimmed_file_links	bigWig-unique	minus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3009					GRCh38	V40	RBM22-BG293LV06-11_Aligned.sortedByCoord.out.bam		ENCORE2	29684
RBM22-BG293LV06-11_quant.sf	ENCODE_DATA/set71/salmon_ENCORE2_file_links	transcript_quantification	transcript quantifications	knockdown followed by RNA-seq	Graveley Lab	3009					GRCh38	V40	RBM22-BG293LV06-11_S101_L002_R1_001.filtered.trimmed.paired.fastq.gz,RBM22-BG293LV06-11_S101_L002_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	29748
RBM22-BG293LV06-12_S102_L002_R1_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set71/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3010	NovaSeq6000	100	paired-ended	1				NT-BG293LV06-1merged_S1_L002_R1_001.filtered.trimmed.paired.fastq.gz,NT-BG293LV06-2merged_S2_L002_R1_001.filtered.trimmed.paired.fastq.gz	ENCORE2	29364
RBM22-BG293LV06-12_S102_L002_R2_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set71/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3010	NovaSeq6000	100	paired-ended	2				NT-BG293LV06-1merged_S1_L002_R2_001.filtered.trimmed.paired.fastq.gz,NT-BG293LV06-2merged_S2_L002_R2_001.filtered.trimmed.paired.fastq.gz	ENCORE2	29428
RBM22-BG293LV06-12_Aligned.sortedByCoord.out.bam	ENCODE_DATA/set71/STAR_ENCORE2_trimmed_file_links	bam	alignments	knockdown followed by RNA-seq	Graveley Lab	3010					GRCh38	V40	RBM22-BG293LV06-12_S102_L002_R1_001.filtered.trimmed.paired.fastq.gz,RBM22-BG293LV06-12_S102_L002_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	29493
RBM22-BG293LV06-12_Signal.UniqueMultiple.strand-.bw	ENCODE_DATA/set71/STAR_ENCORE2_trimmed_file_links	bigWig-all	minus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3010					GRCh38	V40	RBM22-BG293LV06-12_Aligned.sortedByCoord.out.bam		ENCORE2	29557
RBM22-BG293LV06-12_Signal.UniqueMultiple.strand+.bw	ENCODE_DATA/set71/STAR_ENCORE2_trimmed_file_links	bigWig+all	plus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3010					GRCh38	V40	RBM22-BG293LV06-12_Aligned.sortedByCoord.out.bam		ENCORE2	29621
RBM22-BG293LV06-12_Signal.Unique.strand-.bw	ENCODE_DATA/set71/STAR_ENCORE2_trimmed_file_links	bigWig-unique	minus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3010					GRCh38	V40	RBM22-BG293LV06-12_Aligned.sortedByCoord.out.bam		ENCORE2	29685
RBM22-BG293LV06-12_quant.sf	ENCODE_DATA/set71/salmon_ENCORE2_file_links	transcript_quantification	transcript quantifications	knockdown followed by RNA-seq	Graveley Lab	3010					GRCh38	V40	RBM22-BG293LV06-12_S102_L002_R1_001.filtered.trimmed.paired.fastq.gz,RBM22-BG293LV06-12_S102_L002_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	29749