LUC7L RNA-Seq in 293T

Experiment Information (Status: Released)

	BG293LV06-103	BG293LV06-104
idx	0	0
TRCN#_or_BGC#	TRCN0000001197	TRCN0000001197
shRNA_or_gRNA_sequence	CCGGGAGGAAATCAGTGCGGAAGTTCTCGAGAACTTCCGCACTGATTTCCTCTTTTT	CCGGGAGGAAATCAGTGCGGAAGTTCTCGAGAACTTCCGCACTGATTTCCTCTTTTT
PAM
Name	LUC7L	LUC7L
Sample_ID	BG293LV06-103	BG293LV06-104
transduction_Date	9/5/24	9/5/24
days	D4	D4
RBP_name	LUC7L	LUC7L
qPCR_result	89.9	88.9
Ave_qPCR	89.4
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc	0	0
WB_result
Ave_WB
WB_DONE_date
MW
IP
antibody_Cat#
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM	0	0
Rep2_TPM	0	0
Action	Ready	Ready
Library_start_date
repeat_library
Note
ID	12238	12239

Western Blot Western Blot info was not avaliable

Experiment Status

	BG293LV06-103	BG293LV06-104
Sample_ID	BG293LV06-103	BG293LV06-104
Sample Name
Sample Name	LUC7L-BG293LV06-103B	LUC7L-BG293LV06-104B
RBP	LUC7L	LUC7L
Cell_Line	293T	293T
Exp UID
Status	Released	Released
Status_date	2025-04-21	2025-04-21
Project	ENCORE2	ENCORE2
Note
ID	13540	13541

Library-Prep
Sequencing

Library-Prep Information

	BG293LV06-103	BG293LV06-104
Sample #	103	104
Sample Name	LUC7L-BG293LV06-103B	LUC7L-BG293LV06-104B
Sample_Name_Alias
Index Well Position	G06	H06
Index_table	IDT_UniqueDualIndex_96_V2	IDT_UniqueDualIndex_96_V2
LibPrep_date	2024-10-02	2024-10-02
Lib_ID	Lib-241002	Lib-241002
Tecan_Location
Tecan
Tecan_date
Size_bp	278	270
Peak_Molarity	79.60	76.40
libSampleQC_DNA_Well	DNA_Library_set72/set1/G6.png	DNA_Library_set72/set1/H6.png
RIN	9.9	10.0
libSample_RNA_Well	RNA_Library_set72/set1/G6.png	RNA_Library_set72/set1/H6.png
SampleQC_method	TapeStation_2022	TapeStation_2022
SampleQC_date	2024-10-04	2024-10-04
Sample_ID	BG293LV06-103	BG293LV06-104
RBP	LUC7L	LUC7L
Batch_ID	BG293LV06	BG293LV06
WB_result	0.000	0.000
Library Description	TruSeq mRNA	TruSeq mRNA
Repeat_Library_Suffix	B	B
Lib_Status	SendToSequence	SendToSequence
Project	ENCORE2	ENCORE2
ID	4528	4529

Sequencing Information

	BG293LV06-103	BG293LV06-104
Sample_ID	BG293LV06-103	BG293LV06-104
Sample Name	LUC7L-BG293LV06-103B	LUC7L-BG293LV06-104B
Pool ID	Pool-241004	Pool-241004
LocalServer_folder	set72	set72
total_reads	31,906,029	29,301,075
total_aligned_reads	29,972,118	27,182,824
unique_aligned_reads	28,060,833	25,435,695
percent_uniqueAligned	0.87948	0.86808
correlation_replicates	0.995898	0.995898
spikein_reads	113,400	169,338
percent_spikeins	0.00355	0.00578
original_ReadLength	101	101
QC_Status	Submitted	Submitted
ID	3097	3098

Data Submission Information DCC submission information was not avaliable

File Information

file_name	file_path	file_format	output_type	assay_type	lab	Sample ID	platform	read_length	run_type	paired_end	assembly	genome_annotation	derived_from	controlled_by	Project	FILE_ID
LUC7L-BG293LV06-103B_S55_L001_R1_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set72/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3097	NovaSeq6000	100	paired-ended	1				NT-BG293LV06-1Bmerged_S9_L001_R1_001.filtered.trimmed.paired.fastq.gz,NT-BG293LV06-2Bmerged_S10_L001_R1_001.filtered.trimmed.paired.fastq.gz	ENCORE2	29790
LUC7L-BG293LV06-103B_S55_L001_R2_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set72/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3097	NovaSeq6000	100	paired-ended	2				NT-BG293LV06-1Bmerged_S9_L001_R2_001.filtered.trimmed.paired.fastq.gz,NT-BG293LV06-2Bmerged_S10_L001_R2_001.filtered.trimmed.paired.fastq.gz	ENCORE2	29862
LUC7L-BG293LV06-103B_Aligned.sortedByCoord.out.bam	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bam	alignments	knockdown followed by RNA-seq	Graveley Lab	3097					GRCh38	V40	LUC7L-BG293LV06-103B_S55_L001_R1_001.filtered.trimmed.paired.fastq.gz,LUC7L-BG293LV06-103B_S55_L001_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	29934
LUC7L-BG293LV06-103B_Signal.UniqueMultiple.strand-.bw	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bigWig-all	minus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3097					GRCh38	V40	LUC7L-BG293LV06-103B_Aligned.sortedByCoord.out.bam		ENCORE2	30006
LUC7L-BG293LV06-103B_Signal.UniqueMultiple.strand+.bw	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bigWig+all	plus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3097					GRCh38	V40	LUC7L-BG293LV06-103B_Aligned.sortedByCoord.out.bam		ENCORE2	30078
LUC7L-BG293LV06-103B_Signal.Unique.strand-.bw	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bigWig-unique	minus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3097					GRCh38	V40	LUC7L-BG293LV06-103B_Aligned.sortedByCoord.out.bam		ENCORE2	30150
LUC7L-BG293LV06-103B_Signal.Unique.strand+.bw	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bigWig+unique	plus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3097					GRCh38	V40	LUC7L-BG293LV06-103B_Aligned.sortedByCoord.out.bam		ENCORE2	30222
LUC7L-BG293LV06-103B_quant.sf	ENCODE_DATA/set72/salmon_ENCORE2_file_links	transcript_quantification	transcript quantifications	knockdown followed by RNA-seq	Graveley Lab	3097					GRCh38	V40	LUC7L-BG293LV06-103B_S55_L001_R1_001.filtered.trimmed.paired.fastq.gz,LUC7L-BG293LV06-103B_S55_L001_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	30294
LUC7L-BG293LV06-104B_S56_L001_R1_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set72/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3098	NovaSeq6000	100	paired-ended	1				NT-BG293LV06-1Bmerged_S9_L001_R1_001.filtered.trimmed.paired.fastq.gz,NT-BG293LV06-2Bmerged_S10_L001_R1_001.filtered.trimmed.paired.fastq.gz	ENCORE2	29791
LUC7L-BG293LV06-104B_S56_L001_R2_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set72/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3098	NovaSeq6000	100	paired-ended	2				NT-BG293LV06-1Bmerged_S9_L001_R2_001.filtered.trimmed.paired.fastq.gz,NT-BG293LV06-2Bmerged_S10_L001_R2_001.filtered.trimmed.paired.fastq.gz	ENCORE2	29863
LUC7L-BG293LV06-104B_Aligned.sortedByCoord.out.bam	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bam	alignments	knockdown followed by RNA-seq	Graveley Lab	3098					GRCh38	V40	LUC7L-BG293LV06-104B_S56_L001_R1_001.filtered.trimmed.paired.fastq.gz,LUC7L-BG293LV06-104B_S56_L001_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	29935
LUC7L-BG293LV06-104B_Signal.UniqueMultiple.strand-.bw	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bigWig-all	minus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3098					GRCh38	V40	LUC7L-BG293LV06-104B_Aligned.sortedByCoord.out.bam		ENCORE2	30007
LUC7L-BG293LV06-104B_Signal.UniqueMultiple.strand+.bw	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bigWig+all	plus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3098					GRCh38	V40	LUC7L-BG293LV06-104B_Aligned.sortedByCoord.out.bam		ENCORE2	30079
LUC7L-BG293LV06-104B_Signal.Unique.strand-.bw	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bigWig-unique	minus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3098					GRCh38	V40	LUC7L-BG293LV06-104B_Aligned.sortedByCoord.out.bam		ENCORE2	30151
LUC7L-BG293LV06-104B_Signal.Unique.strand+.bw	ENCODE_DATA/set72/STAR_ENCORE2_trimmed_file_links	bigWig+unique	plus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3098					GRCh38	V40	LUC7L-BG293LV06-104B_Aligned.sortedByCoord.out.bam		ENCORE2	30223
LUC7L-BG293LV06-104B_quant.sf	ENCODE_DATA/set72/salmon_ENCORE2_file_links	transcript_quantification	transcript quantifications	knockdown followed by RNA-seq	Graveley Lab	3098					GRCh38	V40	LUC7L-BG293LV06-104B_S56_L001_R1_001.filtered.trimmed.paired.fastq.gz,LUC7L-BG293LV06-104B_S56_L001_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	30295