RBM11 RNA-Seq in 293T

Experiment Information (Status: Submitting)

	BG293LV04-53	BG293LV04-54
idx	0	0
TRCN#_or_BGC#	TRCN0000162312	TRCN0000162312
shRNA_or_gRNA_sequence	CCGGCCCAGCTCATATAAATGGACTCTCGAGAGTCCATTTATATGAGCTGGGTTTTTTG	CCGGCCCAGCTCATATAAATGGACTCTCGAGAGTCCATTTATATGAGCTGGGTTTTTTG
PAM
Name	RBM11	RBM11
Sample_ID	BG293LV04-53	BG293LV04-54
transduction_Date	9/7/23	9/7/23
days	D4	D4
RBP_name	RBM11	RBM11
qPCR_result	79.5	82.2
Ave_qPCR	80.8
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc	0	0
WB_result
Ave_WB
WB_DONE_date
MW
IP
antibody_Cat#
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM	0	0
Rep2_TPM	0	0
Action	Ready	Ready
Library_start_date
repeat_library
Note
ID	9940	9941

Western Blot Western Blot info was not avaliable

Experiment Status

	BG293LV04-53	BG293LV04-54
Sample_ID	BG293LV04-53	BG293LV04-54
Sample Name
Sample Name	RBM11-BG293LV04-53	RBM11-BG293LV04-54
RBP	RBM11	RBM11
Cell_Line	293T	293T
Exp UID
Status	Submitting	Submitting
Status_date	2023-12-30	2023-12-30
Project	ENCORE2	ENCORE2
Note
ID	12532	12533

Library-Prep
Sequencing

Library-Prep Information

	BG293LV04-53	BG293LV04-54
Sample #	19	20
Sample Name	RBM11-BG293LV04-53	RBM11-BG293LV04-54
Sample_Name_Alias
Index Well Position	C03	D03
Index_table	IDT_UniqueDualIndex_96	IDT_UniqueDualIndex_96
LibPrep_date	2023-10-10	2023-10-10
Lib_ID	Lib-231010	Lib-231010
Tecan_Location
Tecan
Tecan_date
Size_bp	274	280
Peak_Molarity	92.90	65.10
libSampleQC_DNA_Well	DNA_Library_set64/set1/C11.png	DNA_Library_set64/set1/D11.png
RIN	9.6	9.5
libSample_RNA_Well	RNA_Library_set64/set1/C7.png	RNA_Library_set64/set1/D7.png
SampleQC_method	TapeStation_2022	TapeStation_2022
SampleQC_date	2023-10-12	2023-10-12
Sample_ID	BG293LV04-53	BG293LV04-54
RBP	RBM11	RBM11
Batch_ID	BG293LV04	BG293LV04
WB_result	0.000	0.000
Library Description	TruSeq mRNA	TruSeq mRNA
Repeat_Library_Suffix
Lib_Status	SendToSequence	SendToSequence
Project	ENCORE2	ENCORE2
ID	4024	4025

Sequencing Information

	BG293LV04-53	BG293LV04-54
Sample_ID	BG293LV04-53	BG293LV04-54
Sample Name	RBM11-BG293LV04-53	RBM11-BG293LV04-54
Pool ID	Pool-231012	Pool-231012
LocalServer_folder	set64	set64
total_reads	24,542,344	21,911,955
total_aligned_reads	22,944,668	19,850,666
unique_aligned_reads	21,217,655	18,407,960
percent_uniqueAligned	0.86453	0.84009
correlation_replicates	0.991404	0.991404
spikein_reads	4,658	3,310
percent_spikeins	0.00019	0.00015
original_ReadLength	100	100
QC_Status	Submitting	Submitting
ID	2633	2634

Data Submission Information DCC submission information was not avaliable

File Information

file_name	file_path	file_format	output_type	assay_type	lab	Sample ID	platform	read_length	run_type	paired_end	assembly	genome_annotation	derived_from	controlled_by	Project	FILE_ID
RBM11-BG293LV04-53_S19_R1_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set64/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	2633	NextSeq2000	100	paired-ended	1				NT-BG293LV04-1_S1_R1_001.filtered.trimmed.paired.fastq.gz,NT-BG293LV04-2_S2_R1_001.filtered.trimmed.paired.fastq.gz	ENCORE2	8700
RBM11-BG293LV04-53_S19_R2_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set64/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	2633	NextSeq2000	100	paired-ended	2				NT-BG293LV04-1_S1_R2_001.filtered.trimmed.paired.fastq.gz,NT-BG293LV04-2_S2_R2_001.filtered.trimmed.paired.fastq.gz	ENCORE2	8728
RBM11-BG293LV04-53_Aligned.sortedByCoord.out.bam	ENCODE_DATA/set64/STAR_ENCORE2_trimmed_file_links	bam	alignments	knockdown followed by RNA-seq	Graveley Lab	2633					GRCh38	V40	RBM11-BG293LV04-53_S19_R1_001.filtered.trimmed.paired.fastq.gz,RBM11-BG293LV04-53_S19_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	9777
RBM11-BG293LV04-53_Signal.Unique.strand+.bw	ENCODE_DATA/set64/STAR_ENCORE2_trimmed_file_links	bigWig+unique	plus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	2633					GRCh38	V40	RBM11-BG293LV04-53/Aligned.sortedByCoord.out.bam		ENCORE2	9805
RBM11-BG293LV04-53_Signal.Unique.strand-.bw	ENCODE_DATA/set64/STAR_ENCORE2_trimmed_file_links	bigWig-unique	minus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	2633					GRCh38	V40	RBM11-BG293LV04-53/Aligned.sortedByCoord.out.bam		ENCORE2	9833
RBM11-BG293LV04-53_Signal.UniqueMultiple.strand+.bw	ENCODE_DATA/set64/STAR_ENCORE2_trimmed_file_links	bigWig+all	plus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	2633					GRCh38	V40	RBM11-BG293LV04-53/Aligned.sortedByCoord.out.bam		ENCORE2	9861
RBM11-BG293LV04-53_Signal.UniqueMultiple.strand-.bw	ENCODE_DATA/set64/STAR_ENCORE2_trimmed_file_links	bigWig-all	minus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	2633					GRCh38	V40	RBM11-BG293LV04-53/Aligned.sortedByCoord.out.bam		ENCORE2	9889
RBM11-BG293LV04-53_quant.sf	ENCODE_DATA/set64/salmon_ENCORE2	transcript_quantification	transcript quantifications	knockdown followed by RNA-seq	Graveley Lab	2633					GRCh38	V40	RBM11-BG293LV04-53_S19_R1_001.filtered.trimmed.paired.fastq.gz,RBM11-BG293LV04-53_S19_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	9917
RBM11-BG293LV04-54_S20_R1_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set64/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	2634	NextSeq2000	100	paired-ended	1				NT-BG293LV04-1_S1_R1_001.filtered.trimmed.paired.fastq.gz,NT-BG293LV04-2_S2_R1_001.filtered.trimmed.paired.fastq.gz	ENCORE2	8701
RBM11-BG293LV04-54_S20_R2_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set64/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	2634	NextSeq2000	100	paired-ended	2				NT-BG293LV04-1_S1_R2_001.filtered.trimmed.paired.fastq.gz,NT-BG293LV04-2_S2_R2_001.filtered.trimmed.paired.fastq.gz	ENCORE2	8729
RBM11-BG293LV04-54_Aligned.sortedByCoord.out.bam	ENCODE_DATA/set64/STAR_ENCORE2_trimmed_file_links	bam	alignments	knockdown followed by RNA-seq	Graveley Lab	2634					GRCh38	V40	RBM11-BG293LV04-54_S20_R1_001.filtered.trimmed.paired.fastq.gz,RBM11-BG293LV04-54_S20_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	9778
RBM11-BG293LV04-54_Signal.Unique.strand+.bw	ENCODE_DATA/set64/STAR_ENCORE2_trimmed_file_links	bigWig+unique	plus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	2634					GRCh38	V40	RBM11-BG293LV04-54/Aligned.sortedByCoord.out.bam		ENCORE2	9806
RBM11-BG293LV04-54_Signal.Unique.strand-.bw	ENCODE_DATA/set64/STAR_ENCORE2_trimmed_file_links	bigWig-unique	minus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	2634					GRCh38	V40	RBM11-BG293LV04-54/Aligned.sortedByCoord.out.bam		ENCORE2	9834
RBM11-BG293LV04-54_Signal.UniqueMultiple.strand+.bw	ENCODE_DATA/set64/STAR_ENCORE2_trimmed_file_links	bigWig+all	plus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	2634					GRCh38	V40	RBM11-BG293LV04-54/Aligned.sortedByCoord.out.bam		ENCORE2	9862
RBM11-BG293LV04-54_Signal.UniqueMultiple.strand-.bw	ENCODE_DATA/set64/STAR_ENCORE2_trimmed_file_links	bigWig-all	minus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	2634					GRCh38	V40	RBM11-BG293LV04-54/Aligned.sortedByCoord.out.bam		ENCORE2	9890
RBM11-BG293LV04-54_quant.sf	ENCODE_DATA/set64/salmon_ENCORE2	transcript_quantification	transcript quantifications	knockdown followed by RNA-seq	Graveley Lab	2634					GRCh38	V40	RBM11-BG293LV04-54_S20_R1_001.filtered.trimmed.paired.fastq.gz,RBM11-BG293LV04-54_S20_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	9918