HNRNPH1 RNA-Seq in 293T

Experiment Information (Status: Submitting)

	BG293LV04-37	BG293LV04-38
idx	0	0
TRCN#_or_BGC#	TRCN0000074596	TRCN0000074596
shRNA_or_gRNA_sequence	CCGGCCTGTGAGAGTACACATTGAACTCGAGTTCAATGTGTACTCTCACAGGTTTTTG	CCGGCCTGTGAGAGTACACATTGAACTCGAGTTCAATGTGTACTCTCACAGGTTTTTG
PAM
Name	HNRNPH1	HNRNPH1
Sample_ID	BG293LV04-37	BG293LV04-38
transduction_Date	9/7/23	9/7/23
days	D4	D4
RBP_name	HNRNPH1	HNRNPH1
qPCR_result	84.8	81.4
Ave_qPCR	83.1
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc	0	0
WB_result
Ave_WB
WB_DONE_date
MW
IP
antibody_Cat#
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM	0	0
Rep2_TPM	0	0
Action	Ready	Ready
Library_start_date
repeat_library
Note
ID	9924	9925

Western Blot Western Blot info was not avaliable

Experiment Status

	BG293LV04-37	BG293LV04-38
Sample_ID	BG293LV04-37	BG293LV04-38
Sample Name
Sample Name	HNRNPH1-BG293LV04-37	HNRNPH1-BG293LV04-38
RBP	HNRNPH1	HNRNPH1
Cell_Line	293T	293T
Exp UID
Status	Submitting	Submitting
Status_date	2023-12-30	2023-12-30
Project	ENCORE2	ENCORE2
Note
ID	12524	12525

Library-Prep
Sequencing

Library-Prep Information

	BG293LV04-37	BG293LV04-38
Sample #	11	12
Sample Name	HNRNPH1-BG293LV04-37	HNRNPH1-BG293LV04-38
Sample_Name_Alias
Index Well Position	C02	D02
Index_table	IDT_UniqueDualIndex_96	IDT_UniqueDualIndex_96
LibPrep_date	2023-10-10	2023-10-10
Lib_ID	Lib-231010	Lib-231010
Tecan_Location
Tecan
Tecan_date
Size_bp	274	275
Peak_Molarity	88.20	79.00
libSampleQC_DNA_Well	DNA_Library_set64/set1/C10.png	DNA_Library_set64/set1/D10.png
RIN	10.0	9.9
libSample_RNA_Well	RNA_Library_set64/set1/C6.png	RNA_Library_set64/set1/D6.png
SampleQC_method	TapeStation_2022	TapeStation_2022
SampleQC_date	2023-10-12	2023-10-12
Sample_ID	BG293LV04-37	BG293LV04-38
RBP	HNRNPH1	HNRNPH1
Batch_ID	BG293LV04	BG293LV04
WB_result	0.000	0.000
Library Description	TruSeq mRNA	TruSeq mRNA
Repeat_Library_Suffix
Lib_Status	SendToSequence	SendToSequence
Project	ENCORE2	ENCORE2
ID	4016	4017

Sequencing Information

	BG293LV04-37	BG293LV04-38
Sample_ID	BG293LV04-37	BG293LV04-38
Sample Name	HNRNPH1-BG293LV04-37	HNRNPH1-BG293LV04-38
Pool ID	Pool-231012	Pool-231012
LocalServer_folder	set64	set64
total_reads	25,415,472	21,391,160
total_aligned_reads	23,379,130	19,721,485
unique_aligned_reads	21,764,253	18,425,159
percent_uniqueAligned	0.85634	0.86134
correlation_replicates	0.989559	0.989559
spikein_reads	9,760	4,850
percent_spikeins	0.00038	0.00023
original_ReadLength	100	100
QC_Status	Submitting	Submitting
ID	2625	2626

Data Submission Information DCC submission information was not avaliable

File Information

file_name	file_path	file_format	output_type	assay_type	lab	Sample ID	platform	read_length	run_type	paired_end	assembly	genome_annotation	derived_from	controlled_by	Project	FILE_ID
HNRNPH1-BG293LV04-37_S11_R1_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set64/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	2625	NextSeq2000	100	paired-ended	1				NT-BG293LV04-1_S1_R1_001.filtered.trimmed.paired.fastq.gz,NT-BG293LV04-2_S2_R1_001.filtered.trimmed.paired.fastq.gz	ENCORE2	8688
HNRNPH1-BG293LV04-37_S11_R2_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set64/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	2625	NextSeq2000	100	paired-ended	2				NT-BG293LV04-1_S1_R2_001.filtered.trimmed.paired.fastq.gz,NT-BG293LV04-2_S2_R2_001.filtered.trimmed.paired.fastq.gz	ENCORE2	8716
HNRNPH1-BG293LV04-37_Aligned.sortedByCoord.out.bam	ENCODE_DATA/set64/STAR_ENCORE2_trimmed_file_links	bam	alignments	knockdown followed by RNA-seq	Graveley Lab	2625					GRCh38	V40	HNRNPH1-BG293LV04-37_S11_R1_001.filtered.trimmed.paired.fastq.gz,HNRNPH1-BG293LV04-37_S11_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	9765
HNRNPH1-BG293LV04-37_Signal.Unique.strand+.bw	ENCODE_DATA/set64/STAR_ENCORE2_trimmed_file_links	bigWig+unique	plus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	2625					GRCh38	V40	HNRNPH1-BG293LV04-37/Aligned.sortedByCoord.out.bam		ENCORE2	9793
HNRNPH1-BG293LV04-37_Signal.Unique.strand-.bw	ENCODE_DATA/set64/STAR_ENCORE2_trimmed_file_links	bigWig-unique	minus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	2625					GRCh38	V40	HNRNPH1-BG293LV04-37/Aligned.sortedByCoord.out.bam		ENCORE2	9821
HNRNPH1-BG293LV04-37_Signal.UniqueMultiple.strand+.bw	ENCODE_DATA/set64/STAR_ENCORE2_trimmed_file_links	bigWig+all	plus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	2625					GRCh38	V40	HNRNPH1-BG293LV04-37/Aligned.sortedByCoord.out.bam		ENCORE2	9849
HNRNPH1-BG293LV04-37_Signal.UniqueMultiple.strand-.bw	ENCODE_DATA/set64/STAR_ENCORE2_trimmed_file_links	bigWig-all	minus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	2625					GRCh38	V40	HNRNPH1-BG293LV04-37/Aligned.sortedByCoord.out.bam		ENCORE2	9877
HNRNPH1-BG293LV04-37_quant.sf	ENCODE_DATA/set64/salmon_ENCORE2	transcript_quantification	transcript quantifications	knockdown followed by RNA-seq	Graveley Lab	2625					GRCh38	V40	HNRNPH1-BG293LV04-37_S11_R1_001.filtered.trimmed.paired.fastq.gz,HNRNPH1-BG293LV04-37_S11_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	9905
HNRNPH1-BG293LV04-38_S12_R1_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set64/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	2626	NextSeq2000	100	paired-ended	1				NT-BG293LV04-1_S1_R1_001.filtered.trimmed.paired.fastq.gz,NT-BG293LV04-2_S2_R1_001.filtered.trimmed.paired.fastq.gz	ENCORE2	8689
HNRNPH1-BG293LV04-38_S12_R2_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set64/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	2626	NextSeq2000	100	paired-ended	2				NT-BG293LV04-1_S1_R2_001.filtered.trimmed.paired.fastq.gz,NT-BG293LV04-2_S2_R2_001.filtered.trimmed.paired.fastq.gz	ENCORE2	8717
HNRNPH1-BG293LV04-38_Aligned.sortedByCoord.out.bam	ENCODE_DATA/set64/STAR_ENCORE2_trimmed_file_links	bam	alignments	knockdown followed by RNA-seq	Graveley Lab	2626					GRCh38	V40	HNRNPH1-BG293LV04-38_S12_R1_001.filtered.trimmed.paired.fastq.gz,HNRNPH1-BG293LV04-38_S12_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	9766
HNRNPH1-BG293LV04-38_Signal.Unique.strand+.bw	ENCODE_DATA/set64/STAR_ENCORE2_trimmed_file_links	bigWig+unique	plus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	2626					GRCh38	V40	HNRNPH1-BG293LV04-38/Aligned.sortedByCoord.out.bam		ENCORE2	9794
HNRNPH1-BG293LV04-38_Signal.Unique.strand-.bw	ENCODE_DATA/set64/STAR_ENCORE2_trimmed_file_links	bigWig-unique	minus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	2626					GRCh38	V40	HNRNPH1-BG293LV04-38/Aligned.sortedByCoord.out.bam		ENCORE2	9822
HNRNPH1-BG293LV04-38_Signal.UniqueMultiple.strand+.bw	ENCODE_DATA/set64/STAR_ENCORE2_trimmed_file_links	bigWig+all	plus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	2626					GRCh38	V40	HNRNPH1-BG293LV04-38/Aligned.sortedByCoord.out.bam		ENCORE2	9850
HNRNPH1-BG293LV04-38_Signal.UniqueMultiple.strand-.bw	ENCODE_DATA/set64/STAR_ENCORE2_trimmed_file_links	bigWig-all	minus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	2626					GRCh38	V40	HNRNPH1-BG293LV04-38/Aligned.sortedByCoord.out.bam		ENCORE2	9878
HNRNPH1-BG293LV04-38_quant.sf	ENCODE_DATA/set64/salmon_ENCORE2	transcript_quantification	transcript quantifications	knockdown followed by RNA-seq	Graveley Lab	2626					GRCh38	V40	HNRNPH1-BG293LV04-38_S12_R1_001.filtered.trimmed.paired.fastq.gz,HNRNPH1-BG293LV04-38_S12_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	9906