RBM14 RNA-Seq in 293T

Experiment Information (Status: Revoked)

	BG293LV02-39	BG293LV02-40
idx	0	0
TRCN#_or_BGC#	TRCN0000072693	TRCN0000072693
shRNA_or_gRNA_sequence	CCGGCCCAGGAGATGATCCTGTTAACTCGAGTTAACAGGATCATCTCCTGGGTTTTTG	CCGGCCCAGGAGATGATCCTGTTAACTCGAGTTAACAGGATCATCTCCTGGGTTTTTG
PAM
Name	RBM14	RBM14
Sample_ID	BG293LV02-39	BG293LV02-40
transduction_Date	3/20/23	3/20/23
days	D4	D4
RBP_name	RBM14	RBM14
qPCR_result	55.3	67.7
Ave_qPCR	61.5
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc	0	0
WB_result
Ave_WB
WB_DONE_date
MW
IP
antibody_Cat#
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM	0	0
Rep2_TPM	0	0
Action	Ready	Ready
Library_start_date
repeat_library
Note
ID	9834	9835

Western Blot Western Blot info was not avaliable

Experiment Status

	BG293LV02-39	BG293LV02-40
Sample_ID	BG293LV02-39	BG293LV02-40
Sample Name
Sample Name	RBM14-BG293LV02-39	RBM14-BG293LV02-40
RBP	RBM14	RBM14
Cell_Line	293T	293T
Exp UID
Status	Revoked	Revoked
Status_date	2023-09-20	2023-09-20
Project	ENCORE2	ENCORE2
Note
ID	11592	11593

Library-Prep
Sequencing

Library-Prep Information

	BG293LV02-39	BG293LV02-40
Sample #	35	36
Sample Name	RBM14-BG293LV02-39	RBM14-BG293LV02-40
Sample_Name_Alias
Index Well Position	C05	D05
Index_table	IDT_UniqueDualIndex_96	IDT_UniqueDualIndex_96
LibPrep_date	2023-04-12	2023-04-12
Lib_ID	Lib-230412	Lib-230412
Tecan_Location
Tecan
Tecan_date
Size_bp	288	282
Peak_Molarity	81.50	74.80
libSampleQC_DNA_Well	DNA_Library_set60-62/set2/C5.png	DNA_Library_set60-62/set2/D5.png
RIN	10.0	9.8
libSample_RNA_Well	RNA_Library_set60-62/set1/C5.png	RNA_Library_set60-62/set1/D5.png
SampleQC_method	TapeStation_2022	TapeStation_2022
SampleQC_date	2023-04-17	2023-04-17
Sample_ID	BG293LV02-39	BG293LV02-40
RBP	RBM14	RBM14
Batch_ID	BG293LV02	BG293LV02
WB_result	0.000	0.000
Library Description	TruSeq mRNA	TruSeq mRNA
Repeat_Library_Suffix
Lib_Status	SendToSequence	SendToSequence
Project	ENCORE2	ENCORE2
ID	3622	3623

Sequencing Information

	BG293LV02-39	BG293LV02-40
Sample_ID	BG293LV02-39	BG293LV02-40
Sample Name	RBM14-BG293LV02-39	RBM14-BG293LV02-40
Pool ID	Pool-230417	Pool-230417
LocalServer_folder	set60	set60
total_reads	63,442,789	22,360,787
total_aligned_reads	57,665,865	14,255,114
unique_aligned_reads	53,464,887	13,241,781
percent_uniqueAligned	0.84273	0.59219
correlation_replicates	0.958732	0.958732
spikein_reads	177,588	31,034
percent_spikeins	0.00280	0.00139
original_ReadLength	101	101
QC_Status	Submitting	Submitting
ID	2493	2494

Data Submission Information DCC submission information was not avaliable

File Information

file_name	file_path	file_format	output_type	assay_type	lab	Sample ID	platform	read_length	run_type	paired_end	assembly	genome_annotation	derived_from	controlled_by	Project	FILE_ID
RBM14-BG293LV02-39_S35_L001_R1_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set60/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	2493	NovaSeq6000	100	paired-ended	1				NT-BG293LV02-1_S1_L001_R1_001.filtered.trimmed.paired.fastq.gz,NT-BG293LV02-2_S2_L001_R1_001.filtered.trimmed.paired.fastq.gz	ENCORE2	7996
RBM14-BG293LV02-39_S35_L001_R2_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set60/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	2493	NovaSeq6000	100	paired-ended	2				NT-BG293LV02-1_S1_L001_R2_001.filtered.trimmed.paired.fastq.gz,NT-BG293LV02-2_S2_L001_R2_001.filtered.trimmed.paired.fastq.gz	ENCORE2	8040
RBM14-BG293LV02-39_Aligned.sortedByCoord.out.bam	ENCODE_DATA/set60/STAR_ENCORE2_trimmed_file_links	bam	alignments	knockdown followed by RNA-seq	Graveley Lab	2493					GRCh38	V40	RBM14-BG293LV02-39_S35_L001_R1_001.filtered.trimmed.paired.fastq.gz,RBM14-BG293LV02-39_S35_L001_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	10789
RBM14-BG293LV02-39_Signal.Unique.strand+.bw	ENCODE_DATA/set60/STAR_ENCORE2_trimmed_file_links	bigWig+unique	plus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	2493					GRCh38	V40	RBM14-BG293LV02-39/Aligned.sortedByCoord.out.bam		ENCORE2	10833
RBM14-BG293LV02-39_Signal.Unique.strand-.bw	ENCODE_DATA/set60/STAR_ENCORE2_trimmed_file_links	bigWig-unique	minus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	2493					GRCh38	V40	RBM14-BG293LV02-39/Aligned.sortedByCoord.out.bam		ENCORE2	10877
RBM14-BG293LV02-39_Signal.UniqueMultiple.strand+.bw	ENCODE_DATA/set60/STAR_ENCORE2_trimmed_file_links	bigWig+all	plus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	2493					GRCh38	V40	RBM14-BG293LV02-39/Aligned.sortedByCoord.out.bam		ENCORE2	10921
RBM14-BG293LV02-39_Signal.UniqueMultiple.strand-.bw	ENCODE_DATA/set60/STAR_ENCORE2_trimmed_file_links	bigWig-all	minus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	2493					GRCh38	V40	RBM14-BG293LV02-39/Aligned.sortedByCoord.out.bam		ENCORE2	10965
RBM14-BG293LV02-39_quant.sf	ENCODE_DATA/set60/salmon_ENCORE2	transcript_quantification	transcript quantifications	knockdown followed by RNA-seq	Graveley Lab	2493					GRCh38	V40	RBM14-BG293LV02-39_S35_L001_R1_001.filtered.trimmed.paired.fastq.gz,RBM14-BG293LV02-39_S35_L001_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	11009
RBM14-BG293LV02-40_S36_L001_R1_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set60/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	2494	NovaSeq6000	100	paired-ended	1				NT-BG293LV02-1_S1_L001_R1_001.filtered.trimmed.paired.fastq.gz,NT-BG293LV02-2_S2_L001_R1_001.filtered.trimmed.paired.fastq.gz	ENCORE2	7997
RBM14-BG293LV02-40_S36_L001_R2_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set60/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	2494	NovaSeq6000	100	paired-ended	2				NT-BG293LV02-1_S1_L001_R2_001.filtered.trimmed.paired.fastq.gz,NT-BG293LV02-2_S2_L001_R2_001.filtered.trimmed.paired.fastq.gz	ENCORE2	8041
RBM14-BG293LV02-40_Aligned.sortedByCoord.out.bam	ENCODE_DATA/set60/STAR_ENCORE2_trimmed_file_links	bam	alignments	knockdown followed by RNA-seq	Graveley Lab	2494					GRCh38	V40	RBM14-BG293LV02-40_S36_L001_R1_001.filtered.trimmed.paired.fastq.gz,RBM14-BG293LV02-40_S36_L001_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	10790
RBM14-BG293LV02-40_Signal.Unique.strand+.bw	ENCODE_DATA/set60/STAR_ENCORE2_trimmed_file_links	bigWig+unique	plus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	2494					GRCh38	V40	RBM14-BG293LV02-40/Aligned.sortedByCoord.out.bam		ENCORE2	10834
RBM14-BG293LV02-40_Signal.Unique.strand-.bw	ENCODE_DATA/set60/STAR_ENCORE2_trimmed_file_links	bigWig-unique	minus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	2494					GRCh38	V40	RBM14-BG293LV02-40/Aligned.sortedByCoord.out.bam		ENCORE2	10878
RBM14-BG293LV02-40_Signal.UniqueMultiple.strand+.bw	ENCODE_DATA/set60/STAR_ENCORE2_trimmed_file_links	bigWig+all	plus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	2494					GRCh38	V40	RBM14-BG293LV02-40/Aligned.sortedByCoord.out.bam		ENCORE2	10922
RBM14-BG293LV02-40_Signal.UniqueMultiple.strand-.bw	ENCODE_DATA/set60/STAR_ENCORE2_trimmed_file_links	bigWig-all	minus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	2494					GRCh38	V40	RBM14-BG293LV02-40/Aligned.sortedByCoord.out.bam		ENCORE2	10966
RBM14-BG293LV02-40_quant.sf	ENCODE_DATA/set60/salmon_ENCORE2	transcript_quantification	transcript quantifications	knockdown followed by RNA-seq	Graveley Lab	2494					GRCh38	V40	RBM14-BG293LV02-40_S36_L001_R1_001.filtered.trimmed.paired.fastq.gz,RBM14-BG293LV02-40_S36_L001_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	11010