ERO1A

General Information

Gene Name       : ERO1A
Gene ID             : ENSG00000197930.12
Essential Gene : No

Functions

Localization

Domains (AlphaFold: ERO1A)


Antibody Validation

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878ERO1AENSG00000197930.1234.2027.2330.72ENCSR000AEF
HepG2ERO1AENSG00000197930.1229.3352.8841.11ENCSR985KAT
K562ERO1AENSG00000197930.1214.3516.8915.62ENCSR000AEO

Assays

2 Released experiments are available on ENCORE database.

Assay TypeExp_NameENCODE IDTarget labelBiosampleStatusLinked AntibodyControlsMethodNote
knockdown followed by RNA-seq ERO1L-BGKcLV15-K562ENCSR353OQPERO1LK562ReleasedENCSR404OHQ   CRISPRKD% = 64.2,71.3
knockdown followed by RNA-seq ERO1L-BGHcLV16-HepG2ENCSR561IMVERO1LHepG2ReleasedENCSR964HKT   CRISPRKD% = 60.6,58.5






  eCLIP  

No eCLIP experiment available



  Antibody Validation  
  GeneName    Antibody_DCC_ID    Product_ID    Lot_ID    Phosphate    HepG2 Primary Validation    K562 Primary Validation    HepG2 Secondary Validation    K562 Secondary Validation  
ERO1AENCAB894YPF7027092000403BGHcLV16-43, Released, ENCODE4BGKcLV15-47, Released, ENCODE4


  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
ERO1LK562CRISPRENCSR353OQPENCSR404OHQERO1L-BGKcLV15-47,ERO1L-BGKcLV15-48NT-BGKcLV15-1,NT-BGKcLV15-264.2,71.3ReleasedENCODE4
ERO1LHepG2CRISPRENCSR561IMVENCSR964HKTERO1L-BGHcLV16-43,ERO1L-BGHcLV16-44NT-BGHcLV16-1,NT-BGHcLV16-260.6,58.5ReleasedENCODE4


  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  

No Localization Information available













RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
ERO1AProduct_ID: 702709
Lot_ID: 2000403
Source: Thermo Fisher
Target Name: ERO1A-human		 ThermoFisher_702709_2000403_ERO1L.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the ERO1L specific antibody, 702709. Lanes 1 and 3 are 2.5% of five million whole cell lysate input. Lanes 2 and 4 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the ERO1L-specific antibody, 702709. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.








RNA Targets

  • eCLIP assay for ERO1A is not avaliable on ENCORE database.



Proteins bound to RNA

  • NO known proteins bind on ERO1A RNA with finished eCLIP experiments on ENCORE database.





eCLIP assay for ERO1A is not avaliable on ENCORE database.






NO known proteins are bound to ERO1A RNA with finished eCLIP experiments on ENCORE database.





eCLIP assay for ERO1A is not avaliable on ENCORE database.



Diffrential Expression

  • The Knockdown/knockout assay for ERO1A is not available on ENCORE database.

  • No RBPs affect the gene expression of ERO1A on ENCORE database.


The Knockdown/knockout RNA-Seq assay for ERO1A is not available on ENCORE database.






No RBPs affect the gene expression of ERO1A on ENCORE database.






The Knockdown/knockout RNA-Seq assay for ERO1A is not available on ENCORE database.






No RBPs affect the alternative splcing events of ERO1A on ENCORE database.





The Knockdown/knockout RNA-Seq assay for ERO1A is not avaliable on ENCORE database.