Caption: IP-Western Blot analysis of HepG2 whole cell lysate using PRPF8 specific antibody. Lane 1 is 3% of ten million whole cell lysate input (lane under '10% input') , lane 2 is 30% of IP enrichment using rabbit normal IgG (lane under 'IgG') and lane 3 is 30% IP enrichment using rabbit monoclonal anti-PRPF8 antibody (lanes under 'anti-PRPF8'). Asterisk indicates non-specific band. Conformation specific secondary antibody (Cell Signaling Technology, #5127) was used for western blot. Method: immunoprecipitation Releated Sample: eCLIP:291 Status: Released Lab: Fu Lab |