PRPF4 antibody: RN093PW


Antibody Info
Product_IDRN093PW
Lot_ID1
Host_organismrabbit
SourceMBLI
Antibody_DCC_IDENCAB624FBH
Target Info
GeneNamePRPF4
Phosphate
Target_namePRPF4-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 HepG2_MBL_RN093PW_001_PRPF4.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using PRPF4 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-PRPF4 antibody (lanes under 'PRPF4').

Caption: IP-Western Blot analysis of HepG2 whole cell lysate using PRPF4 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-PRPF4 antibody (lanes under 'PRPF4').

Method: immunoprecipitation

Releated Sample: eCLIP:752

Status: Released

Lab: Yeo Lab

PRPF4-HEPG2.png<br>Caption: Western blot following shRNA against PRPF4 in HepG2 whole cell lysate using PRPF4 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against PRPF4.PRPF4 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Caption: Western blot following shRNA against PRPF4 in HepG2 whole cell lysate using PRPF4 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against PRPF4.PRPF4 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Releated Sample: BGHLV37-41

Status: Released

Lab: Graveley Lab

K562 PRPF4_K562.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using PRPF4 specific antibody. Lane 1 is 2% of ten million whole cell lysate input (lane under '10% input') , lane 2 is 20% of IP enrichment using rabbit normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using rabbit polyclonal anti-PRPF4 antibody (lanes under 'anti-PRPF4').

Caption: IP-Western Blot analysis of K562 whole cell lysate using PRPF4 specific antibody. Lane 1 is 2% of ten million whole cell lysate input (lane under '10% input') , lane 2 is 20% of IP enrichment using rabbit normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using rabbit polyclonal anti-PRPF4 antibody (lanes under 'anti-PRPF4').

Method: immunoprecipitation

Status: Released

Lab: Fu Lab

PRPF4-K562.png<br>Caption: Western blot following shRNA against PRPF4 in k562 whole cell lysate using PRPF4 specific antibody. Lane 1 is a ladder, lane 2 is k562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against PRPF4.PRPF4 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Caption: Western blot following shRNA against PRPF4 in k562 whole cell lysate using PRPF4 specific antibody. Lane 1 is a ladder, lane 2 is k562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against PRPF4.PRPF4 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Releated Sample: BGKLV38-63

Status: Released

Lab: Graveley Lab