| HepG2 | 
Caption: IP-Western Blot analysis of HepG2 whole cell lysate using SRSF9 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 20% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 20% IP enrichment using rabbit polyclonal anti-SRSF9 antibody (lanes under 'SRSF9'). Method: immunoprecipitation Releated Sample: eCLIP:216 Status: Released Lab: Yeo Lab 
Caption: Representative image of immunoprecipitation performed on whole cell extracts from the HepG2 cell line using the SRSF9-specific antibody RN081PW. Lane 1: Input from IP using control IgG. Lane 2: Immunoprecipitated material using control IgG. Lane 3: Input from IP using SRSF9 antibody. Lane 4: Immunoprecipitated material using SRSF9 antibody. Outlined regions were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 25.54 kDa. Method: immunoprecipitation Releated Sample: eCLIP:216 Status: Released Lab: Yeo Lab | 
Caption: Western blot following CRISPR against SRSF9 in HepG2 whole cell lysate using SRSF9 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SRSF9.SRSF9 protein appears as the green band, Tubulin serves as a control and appears in red. Releated Sample: BGHcLV09-21 Status: Released Lab: Graveley Lab 
Caption: IP followed by mass spectrometry. Protein was immunoprecipitated from HepG2 whole cell lysates using the antibody RN081PW, loaded on a 4-12% NuPAGE Bis-Tris gel, and separated via electrophoresis. Using a reference western blot done in parallel, gel pieces corresponding to the sections indicated were excised and submitted for analysis by the UCSD Biomolecular and Proteomics Mass Spectrometry Facility. Status: NotReviewed Lab: Yeo Lab |
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| K562 | 
Caption: IP-Western Blot analysis of K562 whole cell lysate using SRSF9 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 20% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 20% IP enrichment using rabbit polyclonal anti-SRSF9 antibody (lanes under 'SRSF9'). Method: immunoprecipitation Releated Sample: eCLIP:4132 Status: Released Lab: Yeo Lab | 
Caption: Western blot following CRISPR against SRSF9 in K562 whole cell lysate using SRSF9 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SRSF9. SRSF9 protein appears as the green arrow, Tubulin serves as a control and appears in red arrow. Releated Sample: BGKcLV06-35 Status: Released Lab: Graveley Lab |
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