| HepG2 | 
Caption: IP-Western Blot analysis of HepG2 whole cell lysate using SSB specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-SSB antibody (lanes under 'SSB'). Comments: Steven Blue, Yeo Lab: The smudginess in lane 4 of the HepG2 image is most likely due to overexposure and/or an irregularity in the gel. Method: immunoprecipitation Releated Sample: eCLIP:730 Status: Released Lab: Yeo Lab | 
Caption: Western blot following shRNA against SSB in HepG2 whole cell lysate using SSB specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against SSB. SSB protein appears as the green band, GAPDH serves as a control and appears in red. Releated Sample: BGHLV37-61 Status: Released Lab: Graveley Lab |
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| K562 | 
Caption: IP-Western Blot analysis of K562 whole cell lysate using SSB specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-SSB antibody (lanes under 'SSB'). Comments: Steven Blue, Yeo Lab: The smudginess in lane 4 of the HepG2 image is most likely due to overexposure and/or an irregularity in the gel. Method: immunoprecipitation Releated Sample: eCLIP:738 Status: Released Lab: Yeo Lab | |
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