GRSF1 antibody: RN050PW


Antibody Info
Product_IDRN050PW
Lot_ID1
Host_organismrabbit
SourceMBLI
Antibody_DCC_IDENCAB438AYW
Target Info
GeneNameGRSF1
Phosphate
Target_nameGRSF1-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 HepG2_MBLI_RN050PW_001_GRSF1.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using GRSF1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-GRSF1 antibody (lanes under 'GRSF1').

Caption: IP-Western Blot analysis of HepG2 whole cell lysate using GRSF1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-GRSF1 antibody (lanes under 'GRSF1').

Method: immunoprecipitation

Releated Sample: eCLIP:596

Status: Released

Lab: Yeo Lab

GRSF1_Secondary_Western.png<br>Caption: Western blot following shRNA against GRSF1 in HepG2 whole cell lysate using GRSF1 specific antibody. Lane 1 is a ladder, lane 2 is HpeG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against GRSF1. GRSF1 protein appears as the green band, GAPDH serves as a control and appears in red.

Caption: Western blot following shRNA against GRSF1 in HepG2 whole cell lysate using GRSF1 specific antibody. Lane 1 is a ladder, lane 2 is HpeG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against GRSF1. GRSF1 protein appears as the green band, GAPDH serves as a control and appears in red.

Releated Sample: BGHLV22-17

Status: Released

Lab: Graveley Lab

K562 K562_MBLI_RN050PW_001_GRSF1.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using GRSF1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-GRSF1 antibody (lanes under 'GRSF1').

Caption: IP-Western Blot analysis of K562 whole cell lysate using GRSF1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-GRSF1 antibody (lanes under 'GRSF1').

Method: immunoprecipitation

Status: Released

Lab: Yeo Lab

GRSF1-K562_Secondary_Western.png<br>Caption: Western blot following shRNA against GRSF1 in K562 whole cell lysate using GRSF1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against GRSF1.GRSF1 protein appears as the green band, GAPDH serves as a control and appears in red.

Caption: Western blot following shRNA against GRSF1 in K562 whole cell lysate using GRSF1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against GRSF1.GRSF1 protein appears as the green band, GAPDH serves as a control and appears in red.

Releated Sample: BGKLV29-37

Status: Released

Lab: Graveley Lab