PCBP2 antibody: RN025P


Antibody Info
Product_IDRN025P
Lot_ID1
Host_organismrabbit
SourceMBLI
Antibody_DCC_IDENCAB000BFY
Target Info
GeneNamePCBP2
Phosphate
Target_namePCBP2-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 HepG2_MBLI_RN025P_001_PCBP2.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using PCBP2 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-PCBP2 antibody (lanes under 'PCBP2').

Caption: IP-Western Blot analysis of HepG2 whole cell lysate using PCBP2 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-PCBP2 antibody (lanes under 'PCBP2').

Method: immunoprecipitation

Releated Sample: eCLIP:345

Status: Released

Lab: Yeo Lab

PCBP2-HEPG2_Secondary_Western.png<br>Caption: Western blot following shRNA against PCBP2 in HepG2 whole cell lysate using PCBP2 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against PCBP2. PCBP2 protein appears as the green band, Tubulin serves as a control and appears in red.

Caption: Western blot following shRNA against PCBP2 in HepG2 whole cell lysate using PCBP2 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against PCBP2. PCBP2 protein appears as the green band, Tubulin serves as a control and appears in red.

Releated Sample: BGHLV14-29

Status: Released

Lab: Graveley Lab

K562 K562_MBLI_RN025P_001_PCBP2.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using PCBP2 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-PCBP2 antibody (lanes under 'PCBP2').

Caption: IP-Western Blot analysis of K562 whole cell lysate using PCBP2 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-PCBP2 antibody (lanes under 'PCBP2').

Method: immunoprecipitation

Status: Released

Lab: Yeo Lab

PCBP2_Secondary_Western.png<br>Caption: Western blot following shRNA against PCBP2 in K562 whole cell lysate using PCBP2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against PCBP2. PCBP2 protein appears as the green band, Tubulin serves as a control and appears in red.

Caption: Western blot following shRNA against PCBP2 in K562 whole cell lysate using PCBP2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against PCBP2. PCBP2 protein appears as the green band, Tubulin serves as a control and appears in red.

Releated Sample: BGKLV11-31

Status: Released

Lab: Graveley Lab