| HepG2 | 
Caption: IP-Western Blot analysis of HepG2 whole cell lysate using PCBP1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-PCBP1 antibody (lanes under 'PCBP1'). Method: immunoprecipitation Releated Sample: eCLIP:692 Status: Released Lab: Yeo Lab | 
Caption: Western blot following shRNA against PCBP1 in K562 and HepG2 whole cell lysate using PCBP1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against PCBP1. Lanes 5-7 follow the same pattern, but in HepG2. PCBP1 protein appears as the green band, GAPDH serves as a control and appears in red. Releated Sample: BGKLV11-29 Status: Released Lab: Graveley Lab |
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| K562 | 
Caption: IP-Western blot analysis of K562 whole cell lysate using PCBP1 specific antibody. Lane 1 is 2.5% of 0.5mg input lysate, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal Anti-PCBP1 antibody. The last three lanes are the same pattern, but with IgG control instead of PCBP1 antibody. Method: immunoprecipitation Releated Sample: eCLIP:719 Status: Released Lab: Yeo Lab |
Caption: Western blot following shRNA against PCBP1 in K562 and HepG2 whole cell lysate using PCBP1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against PCBP1. Lanes 5-7 follow the same pattern, but in HepG2. PCBP1 protein appears as the green band, GAPDH serves as a control and appears in red. Releated Sample: BGKLV11-29 Status: Released Lab: Graveley Lab |
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