CXXC1 antibody: PA5-76829


Antibody Info
Product_IDPA5-76829
Lot_IDUC2739251A
Host_organismrabbit
SourceThermo Fisher
Antibody_DCC_IDENCAB216VRJ
Target Info
GeneNameCXXC1
Phosphate
Target_nameCXXC1-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 CXXC1-HEPG2-CRISPR-PA5-76829.png<br>Caption: Western blot following CRISPR against CXXC1 in HepG2 whole cell lysate using CXXC1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against CXXC1. CXXC1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Caption: Western blot following CRISPR against CXXC1 in HepG2 whole cell lysate using CXXC1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against CXXC1. CXXC1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Releated Sample: BGHcLV18-7

Status: Released

Lab: Graveley Lab

K562 ThermoFisher_PA5-76829_UC2739251A_CXXC1.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the CXXC1 specific antibody, PA5-76829. Lane 1 is 2.5% of five million whole cell lysate input. Lanes 2 and 3 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the CXXC1-specific antibody, PA5-76829. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.

Caption: IP-WB analysis of K562 whole cell lysate using the CXXC1 specific antibody, PA5-76829. Lane 1 is 2.5% of five million whole cell lysate input. Lanes 2 and 3 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the CXXC1-specific antibody, PA5-76829. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.

Method: immunoprecipitation

Status: NotSatisfied

Lab: Yeo Lab