| HepG2 | | 
Caption: Western blot following CRISPR against DUS3L in HepG2 whole cell lysate using DUS3L specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DUS3L. DUS3L protein appears as the green arrow, GAPDH serves as a control and appears in red arrow. Releated Sample: BGHcLV18-13 Status: Released Lab: Graveley Lab |
|---|
| K562 | 
Caption: IP-WB analysis of K562 whole cell lysate using the DUS3L specific antibody, PA5-59757. Lane 1 is 2.5% of five million whole cell lysate input. Lanes 2 and 3 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the DUS3L-specific antibody, PA5-59757. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used. Method: immunoprecipitation Status: Released Lab: Yeo Lab | 
Caption: Western blot following CRISPR against DUS3L in K562 whole cell lysate using DUS3L specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DUS3L. DUS3L protein appears as the green arrow, GAPDH serves as a control and appears in red arrow. Releated Sample: BGKcLV17-13 Status: Released Lab: Graveley Lab |
|---|