| HepG2 | | 
Caption: Western blot following CRISPR against KPNB1 in HepG2 whole cell lysate using KPNB1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against KPNB1. KPNB1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow. Releated Sample: BGHcLV14-47 Status: Released Lab: Graveley Lab |
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| K562 | 
Caption: IP-WB analysis of K562 whole cell lysate using the KPNB1 specific antibody, MA3-070. Lanes 1 and 3 are 2.5% of five million whole cell lysate input. Lanes 2 and 4 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the KPNB1-specific antibody, MA3-070. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used. Comments: The bands seen at approximately 55 and 27kDa in lanes 2 and 4 are very likely the result of detection of the IgG heavy and light chains of the antibody used to pull down the protein of interest. Method: immunoprecipitation Status: Released Lab: Yeo Lab | 
Caption: Western blot following CRISPR against KPNB1 in K562 whole cell lysate using KPNB1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against KPNB1. KPNB1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow. Releated Sample: BGKcLV13-45 Status: Released Lab: Graveley Lab |
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