PPIL4 antibody: GTX117014


Antibody Info
Product_IDGTX117014
Lot_ID40555
Host_organismrabbit
SourceGeneTex
Antibody_DCC_IDENCAB535ZKU
Target Info
GeneNamePPIL4
Phosphate
Target_namePPIL4-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 PPIL4_Secondary_Western.png<br>Caption: Western blot following shRNA against PPIL4 in K562 and HepG2 whole cell lysate using PPIL4 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against PPIL4. Lanes 5-8 follow the same pattern, but in HepG2. PPIL4 protein appears as the green band, Tubulin serves as a control and appears in red.

Caption: Western blot following shRNA against PPIL4 in K562 and HepG2 whole cell lysate using PPIL4 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against PPIL4. Lanes 5-8 follow the same pattern, but in HepG2. PPIL4 protein appears as the green band, Tubulin serves as a control and appears in red.

Releated Sample: BGKLV24-45

Status: Released

Lab: Graveley Lab

K562 K562_genetex_GTX117014_40555_PPIL4.jpg<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using PPIL4 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal Anti-PPIL4 antibody (lanes under 'PPIL4').

Caption: IP-Western Blot analysis of K562 whole cell lysate using PPIL4 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal Anti-PPIL4 antibody (lanes under 'PPIL4').

Method: immunoprecipitation

Releated Sample: eCLIP:414

Status: Released

Lab: Yeo Lab

PPIL4_Secondary_Western.png<br>Caption: Western blot following shRNA against PPIL4 in K562 and HepG2 whole cell lysate using PPIL4 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against PPIL4. Lanes 5-8 follow the same pattern, but in HepG2. PPIL4 protein appears as the green band, Tubulin serves as a control and appears in red.

Caption: Western blot following shRNA against PPIL4 in K562 and HepG2 whole cell lysate using PPIL4 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against PPIL4. Lanes 5-8 follow the same pattern, but in HepG2. PPIL4 protein appears as the green band, Tubulin serves as a control and appears in red.

Releated Sample: BGKLV24-45

Status: Released

Lab: Graveley Lab