HepG2 | | ![FUBP1-HEPG2.png<br>Caption: Western blot following shRNA against FUBP1 in HepG2 whole cell lysate using FUBP1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against FUBP1.FUBP1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.](https://graveleylab.cam.uchc.edu/ENCODE/ENCODE_DATA/WesternBlot/FUBP1-HEPG2.png)
Caption: Western blot following shRNA against FUBP1 in HepG2 whole cell lysate using FUBP1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against FUBP1.FUBP1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow. Releated Sample: BGHLV37-31 Status: Released Lab: Graveley Lab |
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K562 | ![GeneTex_GTX115154_40401_FUBP1.png<br>Caption: IP-Western blot analysis of K562 whole cell lysate using FUBP1 specific antibody. Lane 1 and lane 4 are 2.5% of 0.5mg input lysate, lane 2 is 2.5% of supernatant after immunoprecipitation using FUBP1 antibody, Lane 3 is 50% of IP enrichment using rabbit polyclonal FUBP1 antibody and lane 5 is 50% of IP enrichement using rabbit normal IgG control antibody. This antibody passes preliminary validation and will be further pursued for primary and secondary validation.](https://graveleylab.cam.uchc.edu/ENCODE/ENCODE_DATA/WesternBlot_primary/GeneTex_GTX115154_40401_FUBP1.png)
Caption: IP-Western blot analysis of K562 whole cell lysate using FUBP1 specific antibody. Lane 1 and lane 4 are 2.5% of 0.5mg input lysate, lane 2 is 2.5% of supernatant after immunoprecipitation using FUBP1 antibody, Lane 3 is 50% of IP enrichment using rabbit polyclonal FUBP1 antibody and lane 5 is 50% of IP enrichement using rabbit normal IgG control antibody. This antibody passes preliminary validation and will be further pursued for primary and secondary validation. Method: immunoprecipitation Status: Released Lab: Yeo Lab | ![FUBP1-k562.png<br>Caption: Western blot following shRNA against FUBP1 in K562 whole cell lysate using FUBP1 specific antibody. Lane 1 is a ladder, lane 2 is k562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against FUBP1.FUBP1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.](https://graveleylab.cam.uchc.edu/ENCODE/ENCODE_DATA/WesternBlot/FUBP1-k562.png)
Caption: Western blot following shRNA against FUBP1 in K562 whole cell lysate using FUBP1 specific antibody. Lane 1 is a ladder, lane 2 is k562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against FUBP1.FUBP1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow. Releated Sample: BGKLV38-37 Status: Released Lab: Graveley Lab |
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