FUBP1 antibody: GTX115154


Antibody Info
Product_IDGTX115154
Lot_ID40401
Host_organismrabbit
SourceGeneTex
Antibody_DCC_IDENCAB000AUG
Target Info
GeneNameFUBP1
Phosphate
Target_nameFUBP1-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 FUBP1-HEPG2.png<br>Caption: Western blot following shRNA against FUBP1 in HepG2 whole cell lysate using FUBP1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against FUBP1.FUBP1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Caption: Western blot following shRNA against FUBP1 in HepG2 whole cell lysate using FUBP1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against FUBP1.FUBP1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Releated Sample: BGHLV37-31

Status: Released

Lab: Graveley Lab

K562 GeneTex_GTX115154_40401_FUBP1.png<br>Caption: IP-Western blot analysis of K562 whole cell lysate using FUBP1 specific antibody. Lane 1 and lane 4 are 2.5% of 0.5mg input lysate, lane 2 is 2.5% of supernatant after immunoprecipitation using FUBP1 antibody, Lane 3 is 50% of IP enrichment using rabbit polyclonal FUBP1 antibody and lane 5 is 50% of IP enrichement using rabbit normal IgG control antibody. This antibody passes preliminary validation and will be further pursued for primary and secondary validation.

Caption: IP-Western blot analysis of K562 whole cell lysate using FUBP1 specific antibody. Lane 1 and lane 4 are 2.5% of 0.5mg input lysate, lane 2 is 2.5% of supernatant after immunoprecipitation using FUBP1 antibody, Lane 3 is 50% of IP enrichment using rabbit polyclonal FUBP1 antibody and lane 5 is 50% of IP enrichement using rabbit normal IgG control antibody. This antibody passes preliminary validation and will be further pursued for primary and secondary validation.

Method: immunoprecipitation

Status: Released

Lab: Yeo Lab

FUBP1-k562.png<br>Caption: Western blot following shRNA against FUBP1 in K562 whole cell lysate using FUBP1 specific antibody. Lane 1 is a ladder, lane 2 is k562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against FUBP1.FUBP1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Caption: Western blot following shRNA against FUBP1 in K562 whole cell lysate using FUBP1 specific antibody. Lane 1 is a ladder, lane 2 is k562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against FUBP1.FUBP1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Releated Sample: BGKLV38-37

Status: Released

Lab: Graveley Lab