POLR2G antibody: GTX108874


Antibody Info
Product_IDGTX108874
Lot_ID40023
Host_organismrabbit
SourceGeneTex
Antibody_DCC_IDENCAB392AZR
Target Info
GeneNamePOLR2G
Phosphate
Target_namePOLR2G-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 HepG2_Genetex_GTX108874_40023_POLR2G.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using POLR2G specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-POLR2G antibody (lanes under 'POLR2G').

Caption: IP-Western Blot analysis of HepG2 whole cell lysate using POLR2G specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-POLR2G antibody (lanes under 'POLR2G').

Comments: This antibody was given and exemption for K562. The data was clean and the pattern is reliable.

Method: immunoprecipitation

Releated Sample: eCLIP:484

Status: Released

Lab: Yeo Lab

K562 POLR2G-CRISPR-K562.png<br>Caption: Western blot following CRISPR against POLR2G in K562 whole cell lysate using POLR2G specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against POLR2G. POLR2G protein appears as the green arrow, Tubulin serves as a control and appears in red arrow.

Caption: Western blot following CRISPR against POLR2G in K562 whole cell lysate using POLR2G specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against POLR2G. POLR2G protein appears as the green arrow, Tubulin serves as a control and appears in red arrow.

Releated Sample: BGKcLV06-25

Status: Released

Lab: Graveley Lab