EIF2S2 antibody: GTX106484


Antibody Info
Product_IDGTX106484
Lot_ID40135
Host_organismrabbit
SourceGeneTex
Antibody_DCC_IDENCAB485WQI
Target Info
GeneNameEIF2S2
Phosphate
Target_nameEIF2S2-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 HepG2_Genetex_GTX106484_40135_EIF2B.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using EIF2S2 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-EIF2S2 antibody (lanes under 'EIF2S2').

Caption: IP-Western Blot analysis of HepG2 whole cell lysate using EIF2S2 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-EIF2S2 antibody (lanes under 'EIF2S2').

Method: immunoprecipitation

Status: Released

Lab: Yeo Lab

EIF2S2_Secondary_Western.png<br>Caption: Western blot following shRNA against EIF2B in K562 and HepG2 whole cell lysate using EIF2B specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against EIF2B. Lanes 5-8 follow the same pattern, but in HepG2. EIF2B protein appears as the green band, Tubulin serves as a control and appears in red.

Caption: Western blot following shRNA against EIF2B in K562 and HepG2 whole cell lysate using EIF2B specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against EIF2B. Lanes 5-8 follow the same pattern, but in HepG2. EIF2B protein appears as the green band, Tubulin serves as a control and appears in red.

Releated Sample: BGKLV29-27

Status: Released

Lab: Graveley Lab

K562 K562_Genetex_GTX106484_40135_EIF2B.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using EIF2B specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-EIF2B antibody (lanes under 'EIF2B').

Caption: IP-Western Blot analysis of K562 whole cell lysate using EIF2B specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-EIF2B antibody (lanes under 'EIF2B').

Method: immunoprecipitation

Status: Released

Lab: Yeo Lab

EIF2S2_Secondary_Western.png<br>Caption: Western blot following shRNA against EIF2B in K562 and HepG2 whole cell lysate using EIF2B specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against EIF2B. Lanes 5-8 follow the same pattern, but in HepG2. EIF2B protein appears as the green band, Tubulin serves as a control and appears in red.

Caption: Western blot following shRNA against EIF2B in K562 and HepG2 whole cell lysate using EIF2B specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against EIF2B. Lanes 5-8 follow the same pattern, but in HepG2. EIF2B protein appears as the green band, Tubulin serves as a control and appears in red.

Releated Sample: BGKLV29-27

Status: Released

Lab: Graveley Lab