| HepG2 | | 
Caption: Western blot following shRNA against APOBEC3C in K562 or HepG2 whole cell lysate using APOBEC3C specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNA against APOBEC3C. Lanes 5-7 follow the same pattern, but in HepG2. APOBEC3C protein appears as the green band, Tubulin serves as a control and appears in red. Releated Sample: BGKLV25-3 Status: Released Lab: Graveley Lab |
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| K562 | 
Caption: IP-Western Blot analysis of K562 whole cell lysate using APOBEC3C specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal Anti-APOBEC3C antibody (lanes under 'APOBEC3C'). This antibody passes preliminary validation and will be further pursued for primary and secondary validation Method: immunoprecipitation Releated Sample: eCLIP:374 Status: Released Lab: Yeo Lab |
Caption: Western blot following shRNA against APOBEC3C in K562 or HepG2 whole cell lysate using APOBEC3C specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNA against APOBEC3C. Lanes 5-7 follow the same pattern, but in HepG2. APOBEC3C protein appears as the green band, Tubulin serves as a control and appears in red. Releated Sample: BGKLV25-3 Status: Released Lab: Graveley Lab |
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