Cell line | Primary validation | Secondary validation |
---|---|---|
HepG2 | ![]() Caption: IP-Western Blot analysis of HepG2 whole cell lysate using HNRNPH1 specific antibody. Lane 1 is 3% of ten million whole cell lysate input (lane under '15% input') , lane 2 is 20% of IP enrichment using rabbit normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using rabbit polyclonal anti-HNRNPH1 antibody (lanes under 'anti-HNRNPH1'). Asterisk indicates heavy chain of antibody. Method: immunoprecipitation Status: Released Lab: Fu Lab | ![]() Caption: Western blot following CRISPR against HNRNPH1 in HepG2 whole cell lysate using HNRNPH1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against HNRNPH1. HNRNPH1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow. Releated Sample: BGHcLV07-15 Status: Released Lab: Graveley Lab |
K562 | ![]() Caption: IP-Western Blot analysis of K562 whole cell lysate using HNRNPH1 specific antibody. Lane 1 is 3% of ten million whole cell lysate input (lane under '15% input') , lane 2 is 20% of IP enrichment using rabbit normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using rabbit polyclonal anti-HNRNPH1 antibody (lanes under 'anti-HNRNPH1'). Asterisk indicates heavy chain of antibody. Method: immunoprecipitation Status: Released Lab: Fu Lab | ![]() Caption: Western blot following CRISPR against HNRNPH1 in K562 whole cell lysate using HNRNPH1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against HNRNPH1. HNRNPH1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow. Releated Sample: BGKcLV06-15 Status: Released Lab: Graveley Lab |
Hela | ![]() Caption: Immunoprecipitation from HeLa whole cell lysate and analized by western blot analysis uisng rabbit polyclonal to HNRNPH1 Comments: There are multiple immunoreactive bands and the marked band does not contain at least 50% of the total signal in the lane. Method: immunoprecipitation Status: NotSatisfied Lab: Fu Lab |