K562 | ![Bethyl_A305-149A_1_RC3H2.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the RC3H2 specific antibody, A305-149A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the RC3H2-specific antibody, A305-149A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.](https://graveleylab.cam.uchc.edu/ENCODE/ENCODE_DATA/WesternBlot_primary/Bethyl_A305-149A_1_RC3H2.png)
Caption: IP-WB analysis of K562 whole cell lysate using the RC3H2 specific antibody, A305-149A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the RC3H2-specific antibody, A305-149A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used. Method: immunoprecipitation Status: Released Lab: Yeo Lab | |
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