CDV3 antibody: A304-660A


Antibody Info
Product_IDA304-660A
Lot_ID1
Host_organismrabbit
SourceBethyl Labs
Antibody_DCC_IDENCAB772KPZ
Target Info
GeneNameCDV3
Phosphate
Target_nameCDV3-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 CDV3-HEPG2-CRISPR-A304-660A.png<br>Caption: Western blot following CRISPR against CDV3 in HepG2 whole cell lysate using CDV3 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against CDV3. CDV3 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Caption: Western blot following CRISPR against CDV3 in HepG2 whole cell lysate using CDV3 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against CDV3. CDV3 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Releated Sample: BGHcLV29-15

Status: Released

Lab: Graveley Lab

K562 Bethyl_A304-660A_1_CDV3.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the CDV3 specific antibody, A304-660A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the CDV3-specific antibody, A304-660A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.

Caption: IP-WB analysis of K562 whole cell lysate using the CDV3 specific antibody, A304-660A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the CDV3-specific antibody, A304-660A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.

Comments: Immunoprecipitation lane (3) exhibits a large deviation from the expected protein size, which we believe is due to performing the characterization using the Jess Western Blotting system. This system has a documented tendency for some proteins to run differently than with more traditional methods due to their compositions and interactions with the electrophoretic components. The image also closely resembles the example image provided by the vendor but secondary validation will be needed for verification.

Method: immunoprecipitation

Status: Released

Lab: Yeo Lab

 CDV3-K562-CRISPR-A304-660A.png<br>Caption: Western blot following CRISPR against CDV3 in K562 whole cell lysate using CDV3 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against CDV3. CDV3 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Caption: Western blot following CRISPR against CDV3 in K562 whole cell lysate using CDV3 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against CDV3. CDV3 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Releated Sample: BGKcLV28-15

Status: Released

Lab: Graveley Lab