FAM120A antibody: A303-889A


Antibody Info
Product_IDA303-889A
Lot_ID1
Host_organismrabbit
SourceBethyl Labs
Antibody_DCC_IDENCAB468JSB
Target Info
GeneNameFAM120A
Phosphate
Target_nameFAM120A-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 HepG2_Bethyl_A303-889A_1_FAM120A.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using FAM120A specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-FAM120A antibody (lanes under 'FAM120A').

Caption: IP-Western Blot analysis of HepG2 whole cell lysate using FAM120A specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-FAM120A antibody (lanes under 'FAM120A').

Method: immunoprecipitation

Releated Sample: eCLIP:297

Status: Released

Lab: Yeo Lab

FAM120A_Secondary_Western.png<br>Caption: Western blot following shRNA against FAM120A in K562 and HepG2 whole cell lysate using FAM120A specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against FAM120A. Lanes 5-8 follow the same pattern, but in HepG2. FAM120A protein appears as the green band, Tubulin serves as a control and appears in red.

Caption: Western blot following shRNA against FAM120A in K562 and HepG2 whole cell lysate using FAM120A specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against FAM120A. Lanes 5-8 follow the same pattern, but in HepG2. FAM120A protein appears as the green band, Tubulin serves as a control and appears in red.

Releated Sample: BGKLV19-27

Status: Released

Lab: Graveley Lab

K562 K562_Bethyl_A303-889A_1_FAM120A.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using FAM120A specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-FAM120A antibody (lanes under 'FAM120A').

Caption: IP-Western Blot analysis of K562 whole cell lysate using FAM120A specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-FAM120A antibody (lanes under 'FAM120A').

Method: immunoprecipitation

Releated Sample: eCLIP:279

Status: Released

Lab: Yeo Lab

FAM120A_Secondary_Western.png<br>Caption: Western blot following shRNA against FAM120A in K562 and HepG2 whole cell lysate using FAM120A specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against FAM120A. Lanes 5-8 follow the same pattern, but in HepG2. FAM120A protein appears as the green band, Tubulin serves as a control and appears in red.

Caption: Western blot following shRNA against FAM120A in K562 and HepG2 whole cell lysate using FAM120A specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against FAM120A. Lanes 5-8 follow the same pattern, but in HepG2. FAM120A protein appears as the green band, Tubulin serves as a control and appears in red.

Releated Sample: BGKLV19-27

Status: Released

Lab: Graveley Lab