| HepG2 | | 
Caption: Western blot following shRNA against ASCC1 in K562 and HepG2 whole cell lysate using ASCC1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against ASCC1. Lanes 5-8 follow the same pattern, but in HepG2. ASCC1 protein appears as the green band, GAPDH serves as a control and appears in red. Releated Sample: BGKLV08-5 Status: Released Lab: Graveley Lab |
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| K562 | 
Caption: IP-WB characterization of ASCC1 specific antibody in K562 cell line . Lane 1 is 2.5% of five million K562 whole cell lysate Input, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal Rabbitanti-ASCC1Antibody. This antibody passes preliminary validation and will be further pursued for primary and secondary validation. Method: immunoprecipitation Status: Released Lab: Yeo Lab |
Caption: Western blot following shRNA against ASCC1 in K562 and HepG2 whole cell lysate using ASCC1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against ASCC1. Lanes 5-8 follow the same pattern, but in HepG2. ASCC1 protein appears as the green band, GAPDH serves as a control and appears in red. Releated Sample: BGKLV08-5 Status: Released Lab: Graveley Lab |
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