DDX42 antibody: A303-353A


Antibody Info
Product_IDA303-353A
Lot_ID1
Host_organismrabbit
SourceBethyl Labs
Antibody_DCC_IDENCAB455YJZ
Target Info
GeneNameDDX42
Phosphate
Target_nameDDX42-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 HEPG2_BETHYL_A303353A_1_DDX42.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using DDX42 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-DDX42 antibody (lanes under 'DDX42').

Caption: IP-Western Blot analysis of HepG2 whole cell lysate using DDX42 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-DDX42 antibody (lanes under 'DDX42').

Method: immunoprecipitation

Status: Released

Lab: Yeo Lab

DDX42-HEPG2-CRISPR.png<br>Caption: Western blot following CRISPR against DDX42 in HepG2 whole cell lysate using DDX42 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DDX42. DDX42 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Caption: Western blot following CRISPR against DDX42 in HepG2 whole cell lysate using DDX42 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DDX42. DDX42 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Releated Sample: BGHcLV05-11

Status: Released

Lab: Graveley Lab

K562 K562_Bethyl_A303-353A_1_DDX42.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using DDX42 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-DDX42 antibody (lanes under 'DDX42').

Caption: IP-Western Blot analysis of K562 whole cell lysate using DDX42 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-DDX42 antibody (lanes under 'DDX42').

Method: immunoprecipitation

Releated Sample: eCLIP:241

Status: Released

Lab: Yeo Lab

DDX42-K562-CRISPR.png<br>Caption: Western blot following CRISPR against DDX42 in K562 whole cell lysate using DDX42 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DDX42.DDX42 protein appears as the green band, Tubulin serves as a control and appears in red.

Caption: Western blot following CRISPR against DDX42 in K562 whole cell lysate using DDX42 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DDX42.DDX42 protein appears as the green band, Tubulin serves as a control and appears in red.

Releated Sample: BGKcLV08-21

Status: Released

Lab: Graveley Lab