DDX52 antibody: A303-054A


Antibody Info
Product_IDA303-054A
Lot_ID1
Host_organismrabbit
SourceBethyl Labs
Antibody_DCC_IDENCAB607JMR
Target Info
GeneNameDDX52
Phosphate
Target_nameDDX52-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 HepG2_Bethyl_A303-054A_1_DDX52.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using DDX52 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-DDX52 antibody (lanes under 'DDX52').

Caption: IP-Western Blot analysis of HepG2 whole cell lysate using DDX52 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-DDX52 antibody (lanes under 'DDX52').

Method: immunoprecipitation

Releated Sample: eCLIP:642

Status: Released

Lab: Yeo Lab

DDX52_Secondary_Western.png<br>Caption: Western blot following shRNA against DDX52 in K562 and HepG2 whole cell lysate using DDX52 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against DDX52. Lanes 5-7 follow the same pattern, but in HepG2. DDX52 protein appears as the green band, GAPDH serves as a control and appears in red.

Caption: Western blot following shRNA against DDX52 in K562 and HepG2 whole cell lysate using DDX52 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against DDX52. Lanes 5-7 follow the same pattern, but in HepG2. DDX52 protein appears as the green band, GAPDH serves as a control and appears in red.

Releated Sample: BGKLV19-17

Status: Released

Lab: Graveley Lab

K562 K562_Bethyl_A303-054A_1_DDX52.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using DDX52 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-DDX52 antibody (lanes under 'DDX52').

Caption: IP-Western Blot analysis of K562 whole cell lysate using DDX52 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-DDX52 antibody (lanes under 'DDX52').

Method: immunoprecipitation

Releated Sample: eCLIP:681

Status: Released

Lab: Yeo Lab

DDX52_Secondary_Western.png<br>Caption: Western blot following shRNA against DDX52 in K562 and HepG2 whole cell lysate using DDX52 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against DDX52. Lanes 5-7 follow the same pattern, but in HepG2. DDX52 protein appears as the green band, GAPDH serves as a control and appears in red.

Caption: Western blot following shRNA against DDX52 in K562 and HepG2 whole cell lysate using DDX52 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against DDX52. Lanes 5-7 follow the same pattern, but in HepG2. DDX52 protein appears as the green band, GAPDH serves as a control and appears in red.

Releated Sample: BGKLV19-17

Status: Released

Lab: Graveley Lab