HepG2 | ![HepG2_Bethyl_A303-054A_1_DDX52.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using DDX52 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-DDX52 antibody (lanes under 'DDX52').](https://graveleylab.cam.uchc.edu/ENCODE/ENCODE_DATA/WesternBlot_primary/HepG2_Bethyl_A303-054A_1_DDX52.png)
Caption: IP-Western Blot analysis of HepG2 whole cell lysate using DDX52 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-DDX52 antibody (lanes under 'DDX52'). Method: immunoprecipitation Releated Sample: eCLIP:642 Status: Released Lab: Yeo Lab | ![DDX52_Secondary_Western.png<br>Caption: Western blot following shRNA against DDX52 in K562 and HepG2 whole cell lysate using DDX52 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against DDX52. Lanes 5-7 follow the same pattern, but in HepG2. DDX52 protein appears as the green band, GAPDH serves as a control and appears in red.](https://graveleylab.cam.uchc.edu/ENCODE/ENCODE_DATA/WesternBlot/DDX52_Secondary_Western.png)
Caption: Western blot following shRNA against DDX52 in K562 and HepG2 whole cell lysate using DDX52 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against DDX52. Lanes 5-7 follow the same pattern, but in HepG2. DDX52 protein appears as the green band, GAPDH serves as a control and appears in red. Releated Sample: BGKLV19-17 Status: Released Lab: Graveley Lab |
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K562 | ![K562_Bethyl_A303-054A_1_DDX52.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using DDX52 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-DDX52 antibody (lanes under 'DDX52').](https://graveleylab.cam.uchc.edu/ENCODE/ENCODE_DATA/WesternBlot_primary/K562_Bethyl_A303-054A_1_DDX52.png)
Caption: IP-Western Blot analysis of K562 whole cell lysate using DDX52 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-DDX52 antibody (lanes under 'DDX52'). Method: immunoprecipitation Releated Sample: eCLIP:681 Status: Released Lab: Yeo Lab | ![DDX52_Secondary_Western.png<br>Caption: Western blot following shRNA against DDX52 in K562 and HepG2 whole cell lysate using DDX52 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against DDX52. Lanes 5-7 follow the same pattern, but in HepG2. DDX52 protein appears as the green band, GAPDH serves as a control and appears in red.](https://graveleylab.cam.uchc.edu/ENCODE/ENCODE_DATA/WesternBlot/DDX52_Secondary_Western.png)
Caption: Western blot following shRNA against DDX52 in K562 and HepG2 whole cell lysate using DDX52 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against DDX52. Lanes 5-7 follow the same pattern, but in HepG2. DDX52 protein appears as the green band, GAPDH serves as a control and appears in red. Releated Sample: BGKLV19-17 Status: Released Lab: Graveley Lab |
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