HSPD1 antibody: A302-845A


Antibody Info
Product_IDA302-845A
Lot_ID1
Host_organismrabbit
SourceBethyl Labs
Antibody_DCC_IDENCAB679PIU
Target Info
GeneNameHSPD1
Phosphate
Target_nameHSPD1-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 HepG2_Bethyl_A302-845A_1_HSPD1.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using HSPD1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-HSPD1 antibody (lanes under 'HSPD1').

Caption: IP-Western Blot analysis of HepG2 whole cell lysate using HSPD1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-HSPD1 antibody (lanes under 'HSPD1').

Method: immunoprecipitation

Status: Released

Lab: Yeo Lab

HSPD1_Secondary_Western.png<br>Caption: Western blot following shRNA against HSPD1 in HepG2 whole cell lysate using HSPD1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against HSPD1. HSPD1 protein appears as the green band, Tubulin serves as a control and appears in red.

Caption: Western blot following shRNA against HSPD1 in HepG2 whole cell lysate using HSPD1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against HSPD1. HSPD1 protein appears as the green band, Tubulin serves as a control and appears in red.

Releated Sample: BGHLV27-25

Status: Released

Lab: Graveley Lab

K562 K562_Bethyl_A302-845A_1_HSPD1.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using HSPD1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-HSPD1 antibody (lanes under 'HSPD1').

Caption: IP-Western Blot analysis of K562 whole cell lysate using HSPD1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-HSPD1 antibody (lanes under 'HSPD1').

Method: immunoprecipitation

Status: Released

Lab: Yeo Lab

HSPD1-K562_Secondary_Western.png<br>Caption: Western blot following shRNA against HSPD1 in K562 whole cell lysate using HSPD1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against HSPD1.HSPD1 protein appears as the green band, Tubulin serves as a control and appears in red.

Caption: Western blot following shRNA against HSPD1 in K562 whole cell lysate using HSPD1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against HSPD1.HSPD1 protein appears as the green band, Tubulin serves as a control and appears in red.

Releated Sample: BGKLV29-41

Status: Released

Lab: Graveley Lab