RBM34 antibody: A302-293A


Antibody Info
Product_IDA302-293A
Lot_ID1
Host_organismrabbit
SourceBethyl Labs
Antibody_DCC_IDENCAB594ZFC
Target Info
GeneNameRBM34
Phosphate
Target_nameRBM34-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 RBM34_Secondary_Western.png<br>Caption: Western blot following shRNA against RBM34 in K562 and HepG2 whole cell lysate using RBM34 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against RBM34. Lanes 5-7 follow the same pattern, but in HepG2. RBM34 protein appears as the green band, GAPDH serves as a control and appears in red.

Caption: Western blot following shRNA against RBM34 in K562 and HepG2 whole cell lysate using RBM34 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against RBM34. Lanes 5-7 follow the same pattern, but in HepG2. RBM34 protein appears as the green band, GAPDH serves as a control and appears in red.

Releated Sample: BGKLV11-43

Status: Released

Lab: Graveley Lab

K562 K562_Bethyl_A302-293A_1_RBM34.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using RBM34 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-RBM34 antibody (lanes under 'RBM34').

Caption: IP-Western Blot analysis of K562 whole cell lysate using RBM34 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-RBM34 antibody (lanes under 'RBM34').

Method: immunoprecipitation

Status: Released

Lab: Yeo Lab

RBM34_Secondary_Western.png<br>Caption: Western blot following shRNA against RBM34 in K562 and HepG2 whole cell lysate using RBM34 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against RBM34. Lanes 5-7 follow the same pattern, but in HepG2. RBM34 protein appears as the green band, GAPDH serves as a control and appears in red.

Caption: Western blot following shRNA against RBM34 in K562 and HepG2 whole cell lysate using RBM34 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against RBM34. Lanes 5-7 follow the same pattern, but in HepG2. RBM34 protein appears as the green band, GAPDH serves as a control and appears in red.

Releated Sample: BGKLV11-43

Status: Released

Lab: Graveley Lab