HepG2 | ![HepG2_Bethyl_A302-075A_1_PPIG.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using PPIG specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-PPIG antibody (lanes under 'PPIG').](https://graveleylab.cam.uchc.edu/ENCODE/ENCODE_DATA/WesternBlot_primary/HepG2_Bethyl_A302-075A_1_PPIG.png)
Caption: IP-Western Blot analysis of HepG2 whole cell lysate using PPIG specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-PPIG antibody (lanes under 'PPIG'). Method: immunoprecipitation Releated Sample: eCLIP:495 Status: Released Lab: Yeo Lab | ![PPIG_Secondary_Western.png<br>Caption: Western blot following shRNA against PPIG in HepG2 whole cell lysate using PPIG specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against PPIG. PPIG protein appears as the green band, Tubulin serves as a control and appears in red.](https://graveleylab.cam.uchc.edu/ENCODE/ENCODE_DATA/WesternBlot/PPIG_Secondary_Western.png)
Caption: Western blot following shRNA against PPIG in HepG2 whole cell lysate using PPIG specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against PPIG. PPIG protein appears as the green band, Tubulin serves as a control and appears in red. Releated Sample: BGHLV27-43 Status: Released Lab: Graveley Lab |
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K562 | ![K562_Bethyl_A302-075A_1_PPIG.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using PPIG specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-PPIG antibody (lanes under 'PPIG'). This antibody passes preliminary validation and will be further pursued for primary and secondary validation.](https://graveleylab.cam.uchc.edu/ENCODE/ENCODE_DATA/WesternBlot_primary/K562_Bethyl_A302-075A_1_PPIG.png)
Caption: IP-Western Blot analysis of K562 whole cell lysate using PPIG specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-PPIG antibody (lanes under 'PPIG'). This antibody passes preliminary validation and will be further pursued for primary and secondary validation. Method: immunoprecipitation Status: Released Lab: Yeo Lab | ![PPIG-K562_Secondary_Western.png<br>Caption: Western blot following shRNA against PPIG in K562 whole cell lysate using PPIG specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against PPIG.PPIG protein appears as the green band, Tubulin serves as a control and appears in red.](https://graveleylab.cam.uchc.edu/ENCODE/ENCODE_DATA/WesternBlot/PPIG-K562_Secondary_Western.png)
Caption: Western blot following shRNA against PPIG in K562 whole cell lysate using PPIG specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against PPIG.PPIG protein appears as the green band, Tubulin serves as a control and appears in red. Releated Sample: BGKLV29-47 Status: Released Lab: Graveley Lab |
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