PPIG antibody: A302-075A


Antibody Info
Product_IDA302-075A
Lot_ID1
Host_organismrabbit
SourceBethyl Labs
Antibody_DCC_IDENCAB460YIG
Target Info
GeneNamePPIG
Phosphate
Target_namePPIG-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 HepG2_Bethyl_A302-075A_1_PPIG.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using PPIG specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-PPIG antibody (lanes under 'PPIG').

Caption: IP-Western Blot analysis of HepG2 whole cell lysate using PPIG specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-PPIG antibody (lanes under 'PPIG').

Method: immunoprecipitation

Releated Sample: eCLIP:495

Status: Released

Lab: Yeo Lab

PPIG_Secondary_Western.png<br>Caption: Western blot following shRNA against PPIG in HepG2 whole cell lysate using PPIG specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against PPIG. PPIG protein appears as the green band, Tubulin serves as a control and appears in red.

Caption: Western blot following shRNA against PPIG in HepG2 whole cell lysate using PPIG specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against PPIG. PPIG protein appears as the green band, Tubulin serves as a control and appears in red.

Releated Sample: BGHLV27-43

Status: Released

Lab: Graveley Lab

K562 K562_Bethyl_A302-075A_1_PPIG.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using PPIG specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-PPIG antibody (lanes under 'PPIG'). This antibody passes preliminary validation and will be further pursued for primary and secondary validation.

Caption: IP-Western Blot analysis of K562 whole cell lysate using PPIG specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-PPIG antibody (lanes under 'PPIG'). This antibody passes preliminary validation and will be further pursued for primary and secondary validation.

Method: immunoprecipitation

Status: Released

Lab: Yeo Lab

PPIG-K562_Secondary_Western.png<br>Caption: Western blot following shRNA against PPIG in K562 whole cell lysate using PPIG specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against PPIG.PPIG protein appears as the green band, Tubulin serves as a control and appears in red.

Caption: Western blot following shRNA against PPIG in K562 whole cell lysate using PPIG specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against PPIG.PPIG protein appears as the green band, Tubulin serves as a control and appears in red.

Releated Sample: BGKLV29-47

Status: Released

Lab: Graveley Lab