ABCF1 antibody: A301-999A


Antibody Info
Product_IDA301-999A
Lot_ID1
Host_organismrabbit
SourceBethyl Labs
Antibody_DCC_IDENCAB710VED
Target Info
GeneNameABCF1
Phosphate
Target_nameABCF1-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 ABCF1-HEPG2.png<br>Caption: Western blot following shRNA against ABCF1 in HepG2 whole cell lysate using ABCF1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against ABCF1.ABCF1 protein appears as the green band, Tubulin serves as a control and appears in red.

Caption: Western blot following shRNA against ABCF1 in HepG2 whole cell lysate using ABCF1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against ABCF1.ABCF1 protein appears as the green band, Tubulin serves as a control and appears in red.

Releated Sample: BGHLV30-3

Status: Released

Lab: Graveley Lab

K562 K562_Bethyl_A301-999A_1_ABCF1.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using ABCF1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-ABCF1 antibody (lanes under 'ABCF1').

Caption: IP-Western Blot analysis of K562 whole cell lysate using ABCF1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-ABCF1 antibody (lanes under 'ABCF1').

Method: immunoprecipitation

Releated Sample: eCLIP:734

Status: Released

Lab: Yeo Lab

ABCF1-K562.png<br>Caption: Western blot following shRNA against ABCF1 in K562 whole cell lysate using ABCF1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against ABCF1. ABCF1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Caption: Western blot following shRNA against ABCF1 in K562 whole cell lysate using ABCF1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against ABCF1. ABCF1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Releated Sample: BGKLV29-3

Status: Released

Lab: Graveley Lab