| HepG2 | 
Caption: IP-Western Blot analysis of HepG2 whole cell lysate using RDBP specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-RDBP antibody (lanes under 'RDBP'). Method: immunoprecipitation Status: Released Lab: Yeo Lab | 
Caption: Western blot following shRNA against NELFE/RDBP in K562 and HepG2 whole cell lysate using NELFE/RDBP specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against NELFE/RDBP. Lanes 5-7 follow the same pattern, but in HepG2. NELFE/RDBP protein appears as the green band, GAPDH serves as a control and appears in red. Releated Sample: BGKLV11-45 Status: Released Lab: Graveley Lab |
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| K562 | 
Caption: IP-Western Blot analysis of K562 whole cell lysate using RDBP specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-RDBP antibody (lanes under 'RDBP'). Method: immunoprecipitation Status: Released Lab: Yeo Lab |
Caption: Western blot following shRNA against NELFE/RDBP in K562 and HepG2 whole cell lysate using NELFE/RDBP specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against NELFE/RDBP. Lanes 5-7 follow the same pattern, but in HepG2. NELFE/RDBP protein appears as the green band, GAPDH serves as a control and appears in red. Releated Sample: BGKLV11-45 Status: Released Lab: Graveley Lab |
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