EIF3D antibody: A301-758A


Antibody Info
Product_IDA301-758A
Lot_ID1
Host_organismrabbit
SourceBethyl Labs
Antibody_DCC_IDENCAB626JLZ
Target Info
GeneNameEIF3D
Phosphate
Target_nameEIF3D-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 HepG2_Bethyl_A301-758A_1_EIF3D.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using EIF3D specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-EIF3D antibody (lanes under 'EIF3D').

Caption: IP-Western Blot analysis of HepG2 whole cell lysate using EIF3D specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-EIF3D antibody (lanes under 'EIF3D').

Method: immunoprecipitation

Releated Sample: eCLIP:625

Status: Released

Lab: Yeo Lab

EIF3D_Secondary_Western.png<br>Caption: Western blot following shRNA against EIF3D in K562 and HepG2 whole cell lysate using EIF3D specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against EIF3D. Lanes 5-7 follow the same pattern, but in HepG2. EIF3D protein appears as the green band, GAPDH serves as a control and appears in red.

Caption: Western blot following shRNA against EIF3D in K562 and HepG2 whole cell lysate using EIF3D specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against EIF3D. Lanes 5-7 follow the same pattern, but in HepG2. EIF3D protein appears as the green band, GAPDH serves as a control and appears in red.

Releated Sample: BGKLV19-25

Status: Released

Lab: Graveley Lab

K562 EIF3D_Secondary_Western.png<br>Caption: Western blot following shRNA against EIF3D in K562 and HepG2 whole cell lysate using EIF3D specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against EIF3D. Lanes 5-7 follow the same pattern, but in HepG2. EIF3D protein appears as the green band, GAPDH serves as a control and appears in red.

Caption: Western blot following shRNA against EIF3D in K562 and HepG2 whole cell lysate using EIF3D specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against EIF3D. Lanes 5-7 follow the same pattern, but in HepG2. EIF3D protein appears as the green band, GAPDH serves as a control and appears in red.

Releated Sample: BGKLV19-25

Status: Released

Lab: Graveley Lab