HepG2 | ![HepG2_Bethyl_A301-758A_1_EIF3D.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using EIF3D specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-EIF3D antibody (lanes under 'EIF3D').](https://graveleylab.cam.uchc.edu/ENCODE/ENCODE_DATA/WesternBlot_primary/HepG2_Bethyl_A301-758A_1_EIF3D.png)
Caption: IP-Western Blot analysis of HepG2 whole cell lysate using EIF3D specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-EIF3D antibody (lanes under 'EIF3D'). Method: immunoprecipitation Releated Sample: eCLIP:625 Status: Released Lab: Yeo Lab | ![EIF3D_Secondary_Western.png<br>Caption: Western blot following shRNA against EIF3D in K562 and HepG2 whole cell lysate using EIF3D specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against EIF3D. Lanes 5-7 follow the same pattern, but in HepG2. EIF3D protein appears as the green band, GAPDH serves as a control and appears in red.](https://graveleylab.cam.uchc.edu/ENCODE/ENCODE_DATA/WesternBlot/EIF3D_Secondary_Western.png)
Caption: Western blot following shRNA against EIF3D in K562 and HepG2 whole cell lysate using EIF3D specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against EIF3D. Lanes 5-7 follow the same pattern, but in HepG2. EIF3D protein appears as the green band, GAPDH serves as a control and appears in red. Releated Sample: BGKLV19-25 Status: Released Lab: Graveley Lab |
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K562 | | ![EIF3D_Secondary_Western.png<br>Caption: Western blot following shRNA against EIF3D in K562 and HepG2 whole cell lysate using EIF3D specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against EIF3D. Lanes 5-7 follow the same pattern, but in HepG2. EIF3D protein appears as the green band, GAPDH serves as a control and appears in red.](https://graveleylab.cam.uchc.edu/ENCODE/ENCODE_DATA/WesternBlot/EIF3D_Secondary_Western.png)
Caption: Western blot following shRNA against EIF3D in K562 and HepG2 whole cell lysate using EIF3D specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against EIF3D. Lanes 5-7 follow the same pattern, but in HepG2. EIF3D protein appears as the green band, GAPDH serves as a control and appears in red. Releated Sample: BGKLV19-25 Status: Released Lab: Graveley Lab |
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