EIF3G antibody: A301-755A


Antibody Info
Product_IDA301-755A
Lot_ID1
Host_organismrabbit
SourceBethyl Labs
Antibody_DCC_IDENCAB042JJK
Target Info
GeneNameEIF3G
Phosphate
Target_nameEIF3G-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 EIF3G_Secondary_Western.png<br>Caption: Western blot following shRNA against EIF3G in K562 and HepG2 whole cell lysate using EIF3G specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against EIF3G. Lanes 5-7 follow the same pattern, but in HepG2. EIF3G protein appears as the green band, GAPDH serves as a control and appears in red.

Caption: Western blot following shRNA against EIF3G in K562 and HepG2 whole cell lysate using EIF3G specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against EIF3G. Lanes 5-7 follow the same pattern, but in HepG2. EIF3G protein appears as the green band, GAPDH serves as a control and appears in red.

Releated Sample: BGKLV13-71

Status: Released

Lab: Graveley Lab

K562 K562_Bethyl_A301-755A_1_EIF3G.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using EIF3G specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-EIF3G antibody (lanes under 'EIF3G').

Caption: IP-Western Blot analysis of K562 whole cell lysate using EIF3G specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-EIF3G antibody (lanes under 'EIF3G').

Method: immunoprecipitation

Releated Sample: eCLIP:646

Status: Released

Lab: Yeo Lab

EIF3G_Secondary_Western.png<br>Caption: Western blot following shRNA against EIF3G in K562 and HepG2 whole cell lysate using EIF3G specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against EIF3G. Lanes 5-7 follow the same pattern, but in HepG2. EIF3G protein appears as the green band, GAPDH serves as a control and appears in red.

Caption: Western blot following shRNA against EIF3G in K562 and HepG2 whole cell lysate using EIF3G specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against EIF3G. Lanes 5-7 follow the same pattern, but in HepG2. EIF3G protein appears as the green band, GAPDH serves as a control and appears in red.

Releated Sample: BGKLV13-71

Status: Released

Lab: Graveley Lab