PABPC4 antibody: A301-466A


Antibody Info
Product_IDA301-466A
Lot_ID1
Host_organismrabbit
SourceBethyl Labs
Antibody_DCC_IDENCAB596MHX
Target Info
GeneNamePABPC4
Phosphate
Target_namePABPC4-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 HepG2_Bethyl_A301-466A_1_PABPC4.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using PABPC4 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-PABPC4 antibody (lanes under 'PABPC4').

Caption: IP-Western Blot analysis of HepG2 whole cell lysate using PABPC4 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-PABPC4 antibody (lanes under 'PABPC4').

Method: immunoprecipitation

Status: Released

Lab: Yeo Lab

PABPC4_Secondary_Western.png<br>Caption: Western blot following shRNA against PABPC4 in K562 and HepG2 whole cell lysate using PABPC4 specific antibody. Lane 4 is a ladder, lane 1 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against PABPC4. Lanes 5-7 follow the same pattern, but in HepG2. PABPC4 protein appears as the green band, GAPDH serves as a control and appears in red.

Caption: Western blot following shRNA against PABPC4 in K562 and HepG2 whole cell lysate using PABPC4 specific antibody. Lane 4 is a ladder, lane 1 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against PABPC4. Lanes 5-7 follow the same pattern, but in HepG2. PABPC4 protein appears as the green band, GAPDH serves as a control and appears in red.

Releated Sample: BGKLV08-37

Status: Released

Lab: Graveley Lab

K562 K562_Bethyl_A301-466A_1_PABPC4.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using PABPC4 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-PABPC4 antibody (lanes under 'PABPC4').

Caption: IP-Western Blot analysis of K562 whole cell lysate using PABPC4 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-PABPC4 antibody (lanes under 'PABPC4').

Method: immunoprecipitation

Releated Sample: eCLIP:406

Status: Released

Lab: Yeo Lab

PABPC4_Secondary_Western.png<br>Caption: Western blot following shRNA against PABPC4 in K562 and HepG2 whole cell lysate using PABPC4 specific antibody. Lane 4 is a ladder, lane 1 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against PABPC4. Lanes 5-7 follow the same pattern, but in HepG2. PABPC4 protein appears as the green band, GAPDH serves as a control and appears in red.

Caption: Western blot following shRNA against PABPC4 in K562 and HepG2 whole cell lysate using PABPC4 specific antibody. Lane 4 is a ladder, lane 1 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against PABPC4. Lanes 5-7 follow the same pattern, but in HepG2. PABPC4 protein appears as the green band, GAPDH serves as a control and appears in red.

Releated Sample: BGKLV08-37

Status: Released

Lab: Graveley Lab