HepG2 |
Caption: IP-Western Blot analysis of HepG2 whole cell lysate using AKAP1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-AKAP1 antibody (lanes under 'AKAP1'). Method: immunoprecipitation Releated Sample: eCLIP:708 Status: Released Lab: Yeo Lab |
Caption: Western blot following shRNA against AKAP1 in HepG2 whole cell lysate using AKAP1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNA against AKAP1. AKAP1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow. Releated Sample: BGHLV12-3 Status: Released Lab: Graveley Lab |
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K562 |
Caption: IP-Western Blot analysis of K562 whole cell lysate using AKAP1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lanes 4 and 5 are 10% IP enrichment from biological replicates using rabbit polyclonal anti-AKAP1 antibody (lanes under 'AKAP1'). Method: immunoprecipitation Releated Sample: eCLIP:697 Status: Released Lab: Yeo Lab |
Caption: Western blot following shRNA against AKAP1 in K562 whole cell lysate using AKAP1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against AKAP1.AKAP1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow. Releated Sample: BGKLV08_3 Status: Released Lab: Graveley Lab |
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