AKAP1 antibody: A301-379A


Antibody Info
Product_IDA301-379A
Lot_ID1
Host_organismrabbit
SourceBethyl Labs
Antibody_DCC_IDENCAB496QKB
Target Info
GeneNameAKAP1
Phosphate
Target_nameAKAP1-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 HepG2_Bethyl_A301-379A_1_AKAP1.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using AKAP1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-AKAP1 antibody (lanes under 'AKAP1').

Caption: IP-Western Blot analysis of HepG2 whole cell lysate using AKAP1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-AKAP1 antibody (lanes under 'AKAP1').

Method: immunoprecipitation

Releated Sample: eCLIP:708

Status: Released

Lab: Yeo Lab

AKAP1-HEPG2.png<br>Caption: Western blot following shRNA against AKAP1 in HepG2 whole cell lysate using AKAP1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNA against AKAP1. AKAP1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Caption: Western blot following shRNA against AKAP1 in HepG2 whole cell lysate using AKAP1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNA against AKAP1. AKAP1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Releated Sample: BGHLV12-3

Status: Released

Lab: Graveley Lab

K562 K562_Bethyl_A301-379A_1_AKAP1.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using AKAP1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lanes 4 and 5 are 10% IP enrichment from biological replicates using rabbit polyclonal anti-AKAP1 antibody (lanes under 'AKAP1').

Caption: IP-Western Blot analysis of K562 whole cell lysate using AKAP1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lanes 4 and 5 are 10% IP enrichment from biological replicates using rabbit polyclonal anti-AKAP1 antibody (lanes under 'AKAP1').

Method: immunoprecipitation

Releated Sample: eCLIP:697

Status: Released

Lab: Yeo Lab

AKAP1-K562.png<br>Caption: Western blot following shRNA against AKAP1 in K562 whole cell lysate using AKAP1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against AKAP1.AKAP1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Caption: Western blot following shRNA against AKAP1 in K562 whole cell lysate using AKAP1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against AKAP1.AKAP1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Releated Sample: BGKLV08_3

Status: Released

Lab: Graveley Lab