DDX51 antibody: A301-089A


Antibody Info
Product_IDA301-089A
Lot_ID1
Host_organismrabbit
SourceBethyl Labs
Antibody_DCC_IDENCAB820ZVZ
Target Info
GeneNameDDX51
Phosphate
Target_nameDDX51-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 DDX51-HEPG2-CRISPR.png<br>Caption: Western blot following CRISPR against DDX51 in HepG2 whole cell lysate using DDX51 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DDX51. DDX51 protein appears as the green band, Beta-actin serves as a control and appears in red.

Caption: Western blot following CRISPR against DDX51 in HepG2 whole cell lysate using DDX51 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DDX51. DDX51 protein appears as the green band, Beta-actin serves as a control and appears in red.

Releated Sample: BGHcLV12-29

Status: Released

Lab: Graveley Lab

K562 K562_Bethyl_A301-089A_1_DDX51.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using DDX51 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-DDX51 antibody (lanes under 'DDX51').

Caption: IP-Western Blot analysis of K562 whole cell lysate using DDX51 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-DDX51 antibody (lanes under 'DDX51').

Method: immunoprecipitation

Releated Sample: eCLIP:586

Status: Released

Lab: Yeo Lab

DDX51-K562.png<br>Caption: Western blot following shRNA against DDX51 in K562 whole cell lysate using DDX51 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against DDX51. DDX51 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Caption: Western blot following shRNA against DDX51 in K562 whole cell lysate using DDX51 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against DDX51. DDX51 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Releated Sample: BGKLV19-15

Status: Released

Lab: Graveley Lab