RBM25 antibody: A301-068A


Antibody Info
Product_IDA301-068A
Lot_ID1
Host_organismrabbit
SourceBethyl Labs
Antibody_DCC_IDENCAB401CSY
Target Info
GeneNameRBM25
Phosphate
Target_nameRBM25-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 RBM25-HEPG2.png<br>Caption: Western blot following shRNA against RBM25 in HepG2 whole cell lysate using RBM25 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against RBM25. RBM25 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Caption: Western blot following shRNA against RBM25 in HepG2 whole cell lysate using RBM25 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against RBM25. RBM25 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Releated Sample: BGHLV12-65

Status: Released

Lab: Graveley Lab

K562 K562_Bethyl_A301-068A_1_RBM25.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using RBM25 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-RBM25 antibody (lanes under 'RBM25').

Caption: IP-Western Blot analysis of K562 whole cell lysate using RBM25 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-RBM25 antibody (lanes under 'RBM25').

Method: immunoprecipitation

Status: Released

Lab: Yeo Lab

RBM25-K562-CRISPR.png<br>Caption: Western blot following CRISPR against RBM25 in K562 whole cell lysate using RBM25 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RBM25. RBM25 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Caption: Western blot following CRISPR against RBM25 in K562 whole cell lysate using RBM25 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RBM25. RBM25 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Releated Sample: BGKcLV11-83

Status: Released

Lab: Graveley Lab