Caption: IP-WB analysis of K562 whole cell lysate using the PRP16 specific antibody, A300-858A. Lanes 1 and 3 are 2.5% of five million whole cell lysate input. Lanes 2 and 4 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the PRP16-specific antibody, A300-858A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used. Comments: Bands at ~55 and ~28kDa in lanes 2 and 4 correspond to the heavy and light chains of the antibody used for the immunoprecipitation. Method: immunoprecipitation Status: Released Lab: Yeo Lab |