DDX24 antibody: A300-696A


Antibody Info
Product_IDA300-696A
Lot_ID1
Host_organismrabbit
SourceBethyl Labs
Antibody_DCC_IDENCAB988AYG
Target Info
GeneNameDDX24
Phosphate
Target_nameDDX24-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 HepG2_Behtyl_A300-696A_1_DDX24.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using DDX24 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-DDX24 antibody (lanes under 'DDX24').

Caption: IP-Western Blot analysis of HepG2 whole cell lysate using DDX24 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-DDX24 antibody (lanes under 'DDX24').

Method: immunoprecipitation

Status: Released

Lab: Yeo Lab

DDX24_Secondary_Western.png<br>Caption: Western blot following shRNA against DDX24 in K562 and HepG2 whole cell lysate using DDX24 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against DDX24. Lanes 5-8 follow the same pattern, but in HepG2. DDX24 protein appears as the green band, Tubulin serves as a control and appears in red.

Caption: Western blot following shRNA against DDX24 in K562 and HepG2 whole cell lysate using DDX24 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against DDX24. Lanes 5-8 follow the same pattern, but in HepG2. DDX24 protein appears as the green band, Tubulin serves as a control and appears in red.

Releated Sample: BGKLV08-13

Status: Released

Lab: Graveley Lab

K562 K562_Bethyl_A300-696A_1_DDX24.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using DDX24 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-DDX24 antibody (lanes under 'DDX24').

Caption: IP-Western Blot analysis of K562 whole cell lysate using DDX24 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-DDX24 antibody (lanes under 'DDX24').

Method: immunoprecipitation

Releated Sample: eCLIP:483

Status: Released

Lab: Yeo Lab

DDX24_Secondary_Western.png<br>Caption: Western blot following shRNA against DDX24 in K562 and HepG2 whole cell lysate using DDX24 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against DDX24. Lanes 5-8 follow the same pattern, but in HepG2. DDX24 protein appears as the green band, Tubulin serves as a control and appears in red.

Caption: Western blot following shRNA against DDX24 in K562 and HepG2 whole cell lysate using DDX24 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against DDX24. Lanes 5-8 follow the same pattern, but in HepG2. DDX24 protein appears as the green band, Tubulin serves as a control and appears in red.

Releated Sample: BGKLV08-13

Status: Released

Lab: Graveley Lab