DDX17 antibody: A300-509A


Antibody Info
Product_IDA300-509A
Lot_ID2
Host_organismrabbit
SourceBethyl Labs
Antibody_DCC_IDENCAB300RAY
Target Info
GeneNameDDX17
Phosphate
Target_nameDDX17-human

Validation Images
Cell linePrimary validationSecondary validation
K562 Bethyl_A300-509A_2_DDX17.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the DDX17 specific antibody, A300-509A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the DDX17-specific antibody, A300-509A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.

Caption: IP-WB analysis of K562 whole cell lysate using the DDX17 specific antibody, A300-509A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the DDX17-specific antibody, A300-509A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.

Method: immunoprecipitation

Status: Released

Lab: Yeo Lab

 DDX17-K562-CRISPR-A300-509A.png<br>Caption: Western blot following CRISPR against DDX17 in K562 whole cell lysate using DDX17 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DDX17. DDX17 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Caption: Western blot following CRISPR against DDX17 in K562 whole cell lysate using DDX17 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DDX17. DDX17 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Releated Sample: BGKcLV28-69

Status: Released

Lab: Graveley Lab