| HepG2 | | 
Caption: Western blot following shRNA against RBM39 in K562 and HepG2 whole cell lysate using RBM39 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against RBM39. Lanes 5-8 follow the same pattern, but in HepG2. RBM39 protein appears as the green band, GAPDH serves as a control and appears in red. Releated Sample: BGKLV28-43 Status: Released Lab: Graveley Lab |
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| K562 | 
Caption: IP-WB analysis of K562 whole cell lysate using RBM39 specific antibody. Lane 1 is 2.5% of 0.5mg input lysate, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal anti-Caper antibody. This antibody passes preliminary validation and will be further pursued for primary and secondary validation. Comments: This antibody was given and exemption for K562. The data was clean and the pattern is reliable. Method: immunoprecipitation Status: Released Lab: Yeo Lab |
Caption: Western blot following shRNA against RBM39 in K562 and HepG2 whole cell lysate using RBM39 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against RBM39. Lanes 5-8 follow the same pattern, but in HepG2. RBM39 protein appears as the green band, GAPDH serves as a control and appears in red. Releated Sample: BGKLV28-43 Status: Released Lab: Graveley Lab |
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